TRPM7 Mediates Mechanosensitivity in Adult Rat Odontoblasts

Journal of Dental Research
Jong Hwa WonS B Oh

Abstract

Odontoblasts, with their strategic arrangement along the outermost compartment of the dentin-pulp complex, have been suggested to have sensory function. In addition to their primary role in dentin formation, growing evidence shows that odontoblasts are capable of sensing mechanical stimulation. Previously, we found that most odontoblasts express TRPM7, the nonselective mechanosensitive ion channel reported to be critical in Mg2+ homeostasis and dentin mineralization. In line with this finding, we sought to elucidate the functional expression of TRPM7 in odontoblasts by pharmacological approaches and mechanical stimulation. Naltriben, a TRPM7-specific agonist, induced calcium transient in the majority of odontoblasts, which was blocked by TRPM7 blockers such as extracellular Mg2+ and FTY720 in a dose-dependent manner. Mechanical stretch of the odontoblastic membrane with hypotonic solution also induced calcium transient, which was blocked by Gd3+, a nonselective mechanosensitive channel blocker. Calcium transient induced by hypotonic solution was also blocked by high extracellular Mg2+ or FTY720. When TRPM7-mediated calcium transients in odontoblasts were analyzed on the subcellular level, remarkably larger transients were detec...Continue Reading

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Citations

May 19, 2018·Pharmaceuticals·Benedikt FelsAlbrecht Schwab
Jan 30, 2019·International Journal of Molecular Sciences·Mohammad Zakir HossainJunichi Kitagawa
Oct 18, 2018·Journal of Molecular Endocrinology·Aran SonDong Min Shin
May 11, 2019·International Journal of Molecular Sciences·Kihwan LeeGehoon Chung
May 28, 2019·British Dental Journal·Kishor Gulabivala, Yuan-Ling Ng
Nov 14, 2019·Journal of Dental Research·P R LeeS B Oh
Oct 18, 2019·Frontiers in Pharmacology·Christina M A P SchuhSebastian Aguayo
Sep 21, 2018·Frontiers in Cellular Neuroscience·Eduardo Couve, Oliver Schmachtenberg
Dec 10, 2020·Cellular Signalling·Fang PeiZhihe Zhao
Jan 31, 2021·Molecular Neurobiology·Yuhui LuoYong Chen
Jun 24, 2020·Peptides·Richard J Bodnar
May 1, 2021·International Journal of Molecular Sciences·Kristóf KádárÁkos Zsembery
Aug 28, 2020·Archives of Oral Biology·Ji Hyun KimSeog Bae Oh
Nov 17, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Josef LavickyJan Krivanek

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Methods Mentioned

BETA
PCR

Software Mentioned

Graphpad
Zen
Prism
Metafluor
ARRIVE

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