TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling

Journal of Experimental & Clinical Cancer Research : CR
Lu LiuJing Wang

Abstract

The epithelial-mesenchymal transition (EMT) is crucial for metastasis and positively regulated by calcium-related signaling. The melastatin-related transient receptor potential 7 (TRPM7) regulates a non-selective cation channel and promotes cancer metastasis. However, the mechanisms underlying the action of TRPM7 in ovarian cancer are unclear. The expression of TRPM7 and EMT markers (Vimentin, N-cadherin, Twist and E-cadherin) in ovarian cancer samples was detected. TRPM7was knockdown by shRNA in Ovarian cancer cell lines to examine calcium [Ca2+]i, EMT markers and PI3K/AKT markers. Various cellular assays, such as invasion and migration, were performed in vitro, and further confirmed in vivo. TRPM7 expression is negatively correlated with E-cadherin, but positively with N-cadherin, Vimentin and Twist expression in ovarian cancer samples. TRPM7 depletion inhibited the migration and invasion in SKOV3 and OVCAR3 cells. In addition, TRPM7 silencing decreased the lung metastasis of SKOV3 tumors and prolonged the survival of tumor-bearing mice. Similar to that of TRPM7 silencing, treatment with MK886, a potent 5-lipoxygenase inhibitor to reduce TRPM7 expression, and/or BAPTA-AM, an intracellular calcium chelator, significantly mitig...Continue Reading

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Citations

May 24, 2019·Cancer Science·Kazuhiro MorishitaHidenori Ichijo
Aug 23, 2020·Cancers·Jason I E Bruce, Andrew D James
Apr 19, 2019·International Journal of Molecular Sciences·Zhi-Guo ZouRhian M Touyz
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Oct 9, 2020·Journal of Experimental & Clinical Cancer Research : CR·Weiwei ShengMing Dong
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Nov 3, 2020·Frontiers in Pharmacology·Giorgia ChinigòDimitra Gkika
Mar 11, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Keizo HiraishiKatsuya Hirano
Mar 18, 2021·Journal of Experimental & Clinical Cancer Research : CR·Parinya SamartSurapol Issaragrisil
May 1, 2021·Diagnostics·Davide SchiroliLucia Merolle
May 28, 2021·The Kaohsiung Journal of Medical Sciences·Ruo-Fan DongJin-Jin Yu
Jul 22, 2021·The FEBS Journal·Ling WuLiang Zhao
Aug 8, 2021·International Journal of Molecular Sciences·Charlotte Van den EyndeJoris Vriens
Sep 29, 2021·Cell Communication and Signaling : CCS·Tingting LiZhiyong Zeng
Dec 23, 2021·Cellular and Molecular Life Sciences : CMLS·Charlotte Van den EyndeJoris Vriens

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Methods Mentioned

BETA
PCR
electrophoresis
flow cytometry
Fluorescent

Software Mentioned

SPSS
ImageJ

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