TRPV1 expression level in isolectin B₄-positive neurons contributes to mouse strain difference in cutaneous thermal nociceptive sensitivity

Journal of Neurophysiology
Kentaro OnoBrian L Schmidt

Abstract

Differential thermal nociception across inbred mouse strains has genetic determinants. Thermal nociception is largely attributed to the heat/capsaicin receptor transient receptor potential vanilloid 1 (TRPV1); however, the contribution of this channel to the genetics of thermal nociception has not been revealed. In this study we compared TRPV1 expression levels and electrophysiological properties in primary sensory neurons and thermal nociceptive behaviors between two (C57BL/6 and BALB/c) inbred mouse strains. Using immunofluorescence and patch-clamp physiology methods, we demonstrated that TRPV1 expression was significantly higher in isolectin B4 (IB4)-positive trigeminal sensory neurons of C57BL/6 relative to BALB/c; the expression in IB4-negative neurons was similar between the strains. Furthermore, using electrophysiological cell classification (current signature method), we showed differences between the two strains in capsaicin sensitivity in IB4-positive neuronal cell types 2 and 13, which were previously reported as skin nociceptors. Otherwise electrophysiological membrane properties of the classified cell types were similar in the two mouse strains. In publicly available nocifensive behavior data and our own behavior d...Continue Reading

References

Feb 6, 2002·Brain Research Bulletin·Tamio FuruseTsuyoshi Koide
May 25, 2002·The European Journal of Neuroscience·Makoto TsudaKazuhide Inoue
Jul 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·C Jeffery WoodburyBrian M Davis
Aug 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey S MogilPeter W Reeh
Aug 19, 2007·Pain·Christopher S NielsenJennifer R Harris
Oct 20, 2009·Cell·Allan I BasbaumDavid Julius
Sep 8, 2010·Neuroscience Letters·Kentaro OnoKiyotoshi Inenaga
May 19, 2011·Molecular Pain·Susan M HuangMichael J Caterina
Apr 10, 2012·The Journal of Pain : Official Journal of the American Pain Society·Yi YeBrian L Schmidt
Jul 25, 2012·Clinical Genetics·C S NielsenÓ A Steingrímsdóttir
Jun 7, 2014·Acta Neuropathologica Communications·Yi YeBrian L Schmidt

❮ Previous
Next ❯

Citations

Jul 7, 2016·Molecular Therapy. Methods & Clinical Development·Bonnie ReinhartJoseph C Glorioso
Feb 24, 2016·Journal of Endodontics·Lorel E BurnsJennifer L Gibbs
Jan 8, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonathan A CohenDaniel H Kaplan
Jan 5, 2021·Frontiers in Physiology·Michael J M FischerArpad Szallasi
Jan 1, 2021·Frontiers in Pharmacology·Jorge B Pineda-FariasNicole N Scheff
Nov 13, 2020·Pharmacology & Therapeutics·Vipin AroraMan-Kyo Chung
Apr 30, 2021·Frontiers in Immunology·Daniel B LowyGila Moalem-Taylor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.