TRPV3 enhances skin keratinocyte proliferation through EGFR-dependent signaling pathways.

Cell Biology and Toxicology
Yujing WangZhengyu Cao

Abstract

Transient receptor potential vanilloid 3 (TRPV3) is highly expressed in skin keratinocytes where it forms Ca2+-permeable nonselective cation channels to regulate various cutaneous functions. TRPV3 expression is upregulated in many skin disorders. Here, we examined how TRPV3 affects keratinocyte proliferation and investigated the underlying mechanism. Topical application of TRPV3 agonist, carvacrol, increased skin thickness in wild type (WT) mice but not in TRPV3 knockout (KO) mice. Carvacrol promoted proliferation of human keratinocytes HaCaT cells at concentrations ≤ 100 μM, but at 300 μM, it decreased cell viability, suggesting a nonmonotonic proliferative effect. Suppression of TRPV3 expression abolished carvacrol-induced cell proliferation while overexpression of TRPV3 enhanced HaCaT cell proliferation. Carvacrol also stimulated Ca2+ influx and proliferation of primary keratinocytes prepared from WT but not TRPV3 KO mice, suggesting that carvacrol-stimulated cell proliferation was dependent on TRPV3-mediated Ca2+ influx. Mechanistic investigation demonstrated that carvacrol stimulated TGFα release and increased phosphorylation levels of EGFR, PI3K, and NF-κB, effects abolished by suppression of TRPV3 expression and CaMKII i...Continue Reading

References

Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
May 23, 2002·Science·Andrea M PeierArdem Patapoutian
Jun 22, 2002·Nature·Haoxing XuDavid E Clapham
Dec 5, 2003·Nature·David E Clapham
Jun 15, 2004·The Journal of Biological Chemistry·Hong-Zhen HuMichael X Zhu
Feb 22, 2005·The Journal of Biological Chemistry·Man-Kyo ChungMichael J Caterina
Jul 22, 2006·The Journal of Investigative Dermatology·Makoto AsakawaTsuneaki Sakata
Dec 13, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Chiang-Wen LeeChuen-Mao Yang
Apr 11, 2007·British Journal of Pharmacology·A K Vogt-EiseleH Hatt
Dec 1, 2007·The Journal of Investigative Dermatology·Saveria PastoreGiampiero Girolomoni
Mar 13, 2008·British Journal of Pharmacology·S P LeeR W Bryant
Mar 24, 2009·Current Neuropharmacology·Joris VriensRudi Vennekens
May 26, 2009·Journal of Inflammation·Kinichi ImuraTsuneaki Sakata
May 20, 2011·The Journal of Investigative Dermatology·István BorbíróTamás Bíró
Jun 30, 2011·Nature Communications·Takashi MiyamotoArdem Patapoutian
Dec 16, 2011·The Journal of Investigative Dermatology·Francesca MasciaStuart H Yuspa
Dec 23, 2011·The Journal of Investigative Dermatology·Mathias SulkMartin Steinhoff
Mar 13, 2012·American Journal of Human Genetics·Zhimiao LinYong Yang
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Oct 30, 2013·Experimental Dermatology·Erika Yamamoto-KasaiTakeshi Yoshioka
Oct 30, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Reona AijimaMizuho A Kido
Aug 20, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Hye One KimJun Young Lee
Aug 8, 2017·The Journal of Clinical Investigation·Loren J MartinLuda Diatchenko
Oct 2, 2017·The Journal of Investigative Dermatology·Attila Gábor SzöllősiTamás Bíró
Mar 20, 2018·The Journal of Investigative Dermatology·Ágnes KeményRolland Gyulai
May 6, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Guoling YangZhengyu Cao

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.