TRPV4 channel participates in receptor-operated calcium entry and ciliary beat frequency regulation in mouse airway epithelial cells.

Proceedings of the National Academy of Sciences of the United States of America
Ivan M LorenzoMiguel A Valverde

Abstract

The rate of mucociliary clearance in the airways is a function of ciliary beat frequency (CBF), and this, in turn, is increased by increases in intracellular calcium. The TRPV4 cation channel mediates Ca(2+) influx in response to mechanical and osmotic stimuli in ciliated epithelia. With the use of a TRPV4-deficient mouse, we now show that TRPV4 is involved in the airways' response to physiologically relevant physical and chemical stimuli. Ciliary TRPV4 expression in tracheal epithelial cells was confirmed with immunofluorescence in TRPV4(+/+) mice. Ciliated tracheal cells from TRPV4(-/-) mice showed no increases in intracellular Ca(2+) and CBF in response to the synthetic activator 4alpha-phorbol 12,13-didecanoate (4alphaPDD) and reduced responses to mild temperature, another TRPV4-activating stimulus. Autoregulation of CBF in response to high viscosity solutions is preserved in TRPV4(-/-) despite a reduced Ca(2+) signal. More interestingly, TRPV4 contributed to an ATP-induced increase in CBF, providing a pathway for receptor-operated Ca(2+) entry but not store-operated Ca(2+) entry as the former mechanism is lost in TRPV4(-/-) cells. Collectively, these results suggest that TRPV4 is predominantly located in the cilia of trach...Continue Reading

References

Nov 1, 1991·The American Review of Respiratory Disease·N T JohnsonP Verdugo
Jan 1, 1990·Annual Review of Physiology·P Satir, M A Sleigh
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·E VázquezM A Valverde
Oct 22, 2002·American Journal of Physiology. Cell Physiology·José M Fernández-FernándezMiguel A Valverde
Nov 27, 2002·The Journal of General Physiology·Weiyuan MaZvi Priel
Mar 6, 2003·Cellular Signalling·Xowi K M S MwimbiAnil Mehta
Jul 31, 2003·Journal of Microscopy·J H SissonT A Wyatt
Sep 2, 2003·Biochimica Et Biophysica Acta·Erik M Schwiebert, Akos Zsembery
Sep 13, 2003·British Journal of Pharmacology·Feng XuToshihiko Iijima
Oct 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wolfgang Liedtke, Jeffrey M Friedman
Jan 7, 2004·American Journal of Physiology. Cell Physiology·Bernd NiliusThomas Voets
Sep 1, 1994·Trends in Cell Biology·S Tamm
Oct 19, 2004·The Journal of Biological Chemistry·Maite ArnigesMiguel A Valverde
Mar 9, 2005·The Journal of Cell Biology·Yaniré N AndradeMiguel A Valverde
Apr 29, 2005·Molecular Reproduction and Development·Stefan C TeilmannSøren T Christensen
Aug 10, 2005·The Journal of Biological Chemistry·Robert TarranRichard C Boucher
Sep 28, 2005·The Journal of Biological Chemistry·Zheng ZhangEmily R Liman
Oct 19, 2005·The Journal of General Physiology·Jason LeeChou-Long Huang
Nov 19, 2005·The Journal of Biological Chemistry·Maite ArnigesMiguel A Valverde
Feb 8, 2006·Annual Review of Physiology·Kimberley B Craven, William N Zagotta
Apr 7, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicole Alessandri-HaberJon D Levine
Jun 7, 2006·The Journal of Biological Chemistry·Seiko F OkadaRichard C Boucher
Jul 28, 2006·The Journal of Biological Chemistry·José J LópezJuan A Rosado
Aug 15, 2006·Nature Cell Biology·Guo N HuangPaul F Worley
Sep 2, 2006·Annual Review of Physiology·Matthias Salathe
Dec 19, 2006·Seminars in Cell & Developmental Biology·Esther Vázquez, Miguel A Valverde
Jan 16, 2007·Handbook of Experimental Pharmacology·M TrebakJ W Putney
Mar 8, 2007·The Journal of Biological Chemistry·Joris VriensBernd Nilius
Jun 29, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Viktor LukacsTibor Rohacs
Oct 20, 2007·The Journal of Clinical Investigation·Thomas GevaertBernd Nilius
Oct 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xibao LiuIndu S Ambudkar
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sergio A GradiloneNicholas F Larusso
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yanhong LiaoLutz Birnbaumer
Apr 2, 2008·The Journal of Cell Biology·Jacqueline FernandesMiguel A Valverde

❮ Previous
Next ❯

Citations

Aug 25, 2009·Pflügers Archiv : European journal of physiology·Carole JungMiguel A Valverde
Oct 12, 2012·Pflügers Archiv : European journal of physiology·Wen-Er LiQing-Hua Liu
Apr 12, 2011·Nature Cell Biology·Kenta FujiuKenjiro Yoshimura
Jul 8, 2010·The Journal of Biological Chemistry·Stephen LoukinChing Kung
Jul 30, 2011·The Journal of Biological Chemistry·Miguel A ValverdeRubén Vicente
Sep 17, 2010·Molecular Biology of the Cell·Panteleimon RompolasStephen M King
Jan 17, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Marc W FarissAndrew J Ghio
Oct 1, 2010·Journal of Cardiovascular Pharmacology·David X Zhang, David D Gutterman
Oct 22, 2010·American Journal of Physiology. Cell Physiology·Taras LyubchenkoEvgenia V Gerasimovskaya
Mar 31, 2012·American Journal of Respiratory Cell and Molecular Biology·Robrecht LembrechtsDirk Adriaensen
Mar 27, 2012·Respiratory Research·Lidwien A M SmitFrancine Kauffmann
Jan 10, 2012·Development·Edwin C Oh, Nicholas Katsanis
Jan 20, 2011·Environmental Health Perspectives·Jinju LiWolfgang Liedtke
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anna Garcia-EliasMiguel A Valverde
Aug 12, 2014·Pharmacology & Therapeutics·Andrea OlschewskiHorst Olschewski
Jun 18, 2014·American Journal of Respiratory Cell and Molecular Biology·Linbo LiuGuillermo J Tearney
Feb 20, 2010·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·J Ulrich SommerBoris A Stuck
Sep 19, 2015·Journal of Cellular Physiology·Julio C SánchezLaura V Muñoz
Jun 26, 2012·European Journal of Cell Biology·Arthur J KuipersFrank N van Leeuwen
May 18, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Maryline BossusCatherine Lorin-Nebel
Mar 23, 2011·Pharmacology & Therapeutics·Katharine Helen BannerChris Poll
Oct 20, 2009·Progress in Biophysics and Molecular Biology·Wouter EveraertsGrzegorz Owsianik
May 16, 2009·Journal of Theoretical Biology·N J WarrenE J Crampin
Dec 17, 2008·Annals of the New York Academy of Sciences·Wolfgang Liedtke
Mar 16, 2011·Biology of the Cell·Andreas Schmid, Matthias Salathe
Nov 30, 2013·British Journal of Pharmacology·M S GraceM G Belvisi
Sep 17, 2013·Basic & Clinical Pharmacology & Toxicology·Lisa AlenmyrPeter M Zygmunt
Mar 13, 2014·Biochimica Et Biophysica Acta·Maxime GuéguinouMarie Potier-Cartereau
May 22, 2010·Journal of Theoretical Biology·N J WarrenE J Crampin
Jul 19, 2016·The International Journal of Biochemistry & Cell Biology·W G DarbyP McIntyre
Jan 17, 2009·Current Topics in Developmental Biology·Gregory J Pazour, Robert A Bloodgood
Feb 4, 2012·Biology of Reproduction·Katharina NoreikatSabine Kölle
Apr 20, 2017·The Journal of Physiology·Karla DroguettNelson P Barrera
Dec 16, 2017·Journal of Cellular Biochemistry·Alejandro PizzoniPaula Ford
Nov 23, 2018·Essays in Biochemistry·Jacob T CroftJohanna L Höög
Jan 12, 2019·The Journal of Pathology·Cheng LiJian Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.