TRPV4 participates in the establishment of trailing adhesions and directional persistence of migrating cells

Pflügers Archiv : European journal of physiology
Sanela MrkonjicMiguel A Valverde

Abstract

Calcium signaling participates in different cellular processes leading to cell migration. TRPV4, a non-selective cation channel that responds to mechano-osmotic stimulation and heat, is also involved in cell migration. However, the mechanistic involvement of TRPV4 in cell migration is currently unknown. We now report that expression of the mutant channel TRPV4-(121)AAWAA (lacking the phosphoinositide-binding site (121)KRWRK(125) and the response to physiological stimuli) altered HEK293 cell migration. Altered migration patterns included periods of fast and persistent motion followed by periods of stalling and turning, and the extension of multiple long cellular protrusions. TRPV4-WT overexpressing cells showed almost complete loss of directionality with frequent turns, no progression, and absence of long protrusions. Traction microscopy revealed higher tractions forces in the tail of TRPV4-(121)AAWAA than in TRPV4-WT expressing cells. These results are consistent with a defective and augmented tail retraction in TRPV4-(121)AAWAA- and TRPV4-WT-expressing cells, respectively. The activity of calpain, a protease implicated in focal adhesion (FA) disassembly, was decreased in TRPV4-(121)AAWAA compared with TRPV4-WT-expressing cells...Continue Reading

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Citations

Apr 12, 2016·Neurology. Genetics·Jeremy M SullivanCharlotte J Sumner
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Carlos Pardo-PastorMiguel A Valverde
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Jan 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Christopher L GilchristFarshid Guilak
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Nov 3, 2020·Frontiers in Pharmacology·Giorgia ChinigòDimitra Gkika
Mar 6, 2021·Nature Communications·Brett A McCrayCharlotte J Sumner
Apr 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Rei NishimotoMakoto Tominaga
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Aug 7, 2021·Biomolecules·Tytti Kärki, Sari Tojkander
Aug 31, 2021·Cells & Development·Brenda Canales Coutiño, Roberto Mayor

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