Trueness, precision and stability of the LIAISON 1-84 parathyroid hormone (PTH) third-generation assay: comparison to existing intact PTH assays.

Clinical Chemistry and Laboratory Medicine : CCLM
Andre ValcourFabrizio Bonelli

Abstract

Over the past few decades, parathyroid hormone (PTH) immunoassays have progressed through successive generations resulting in increased specificity and accuracy for detecting circulating PTH. With the introduction of third-generation assays, in which the biologically active PTH(1-84) is specifically targeted, the PTH(7-84) and other fragments are not detected. The specific recognition of only PTH(1-84) whole molecule allows for more reliable standardization and calibration than with the existing assays. Samples from patients on hemodialysis or with primary hyperparathyroidism and apparently healthy subjects were examined in different collection matrices (EDTA plasma, unspun EDTA plasma and SST) stored for 0, 24 or 72 h at room temperature to reflect the prevailing sample collection methods, shipping and processing conditions of centralized labs in the United States. Samples were analyzed by the LIAISON 1-84 PTH and N-TACT assays, and by three additional commercially available intact PTH assays. Defined samples, prepared using two different standards (WHO 95/646 international standard and the synthetic Bachem PTH(1-84)), show little bias with the LIAISON 1-84 PTH assay, but not with the other intact PTH assays. Furthermore, PTH ...Continue Reading

References

Aug 1, 1983·Canadian Journal of Physiology and Pharmacology·D E Agwu, B J Holub
Jun 22, 2006·Kidney International·J-C SouberbielleP Houillier
Sep 28, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Elodie A HanonEdmund J Lamb
Jun 3, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Etienne CavalierJean-Claude Souberbielle
Oct 18, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Catharine M SturgeonUNKNOWN IFCC Working Group for PTH

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