Truncated and microparticle-free soluble tissue factor bound to peripheral monocytes preferentially activate factor VII

Thrombosis and Haemostasis
Mohammad M H KhanRobert W Colman

Abstract

Soluble plasma tissue factor (TF) circulates in picomolar concentrations in healthy individuals and increases in a wide spectrum of diseases. This study tests the hypothesis that both truncated TF (rsTF) or soluble plasmaTF (pTF) in low concentration combine with monocytes or platelets to convert factorVII (fVII) to fVIIa. Both rsTF (33 kDa) and pTF (47 kDa), obtained from pericardial wounds of patients having cardiac surgery using cardiopulmonary bypass (CPB), were studied in association with blood cells and TF-bearing microparticles. Tissue factor was measured by ELISA. RsTF binds to erythrocytes, platelets, mononuclear cells and polymorphoneutrophils. The rate of fVII conversion with rsTF (1-10(3) nM) is highest with mononuclear cells, less with platelets, minimal with polymorphoneutrophils and undetectable with erythrocytes. Either stimulated or unstimulated mononuclear cells or platelets in the presence of 3.5 pM rsTF or pTF convert fVII (10 pM) [corrected] to fVIIa, but the amounts of fVIIa produced differ. When leukocytes or platelets are absent, microparticles associated with 3.5 pM TF antigen derived from pericardial wound plasma do not activate fVII. Stimulated mononuclear cells convert nearly all available fVII (10 n...Continue Reading

Citations

Mar 15, 2006·The Journal of Experimental Medicine·Robert W Colman
Nov 27, 2007·Journal of Cardiothoracic Surgery·Oliver J WarrenThanos Athanasiou
Dec 8, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Mohammad M KhanRobert W Colman
Oct 16, 2015·Biomarkers in Cancer·Shosaku NomuraMichiomi Shimizu
Mar 14, 2019·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Michael CaspersMarc Maegele
Feb 24, 2015·Journal of Intensive Care·Shosaku Nomura, Michiomi Shimizu
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Valéry DaubiePierre Philippart
Apr 16, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Colin A KretzPeter L Gross
Jan 25, 2008·Journal of Thrombosis and Thrombolysis·Maryam Yavari, Richard C Becker
Mar 20, 2009·Current Opinion in Anaesthesiology·Jean-François HardyPhilippe Van der Linden
Oct 18, 2011·Anesthesia and Analgesia·Roman M Sniecinski, Wayne L Chandler
Dec 7, 2017·Frontiers in Cardiovascular Medicine·Maria T K ZaldiviaKarlheinz Peter
Sep 29, 2015·Journal of Atherosclerosis and Thrombosis·Shosaku Nomura
Jan 1, 2012·Scientifica·Saulius Butenas
Jan 29, 2017·International Journal of Hematology·Shosaku Nomura
Mar 23, 2018·Interactive Cardiovascular and Thoracic Surgery·Aly Makram HabibMichele Di Mauro
Feb 25, 2021·Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine·Julia K BöhmMichael Caspers
Nov 28, 2006·The Annals of Thoracic Surgery·L Henry Edmunds, Robert W Colman
Oct 30, 2007·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·O J WarrenN J W Cheshire
Aug 1, 2008·Thrombosis Research·Shosaku NomuraYasuo Ikeda
Oct 22, 2008·Journal of Cardiothoracic and Vascular Anesthesia·Oliver J WarrenThanos Athanasiou
Aug 4, 2009·Thrombosis Research·Vladimir Y Bogdanov, Bjarne Osterud
Jan 28, 2010·The Annals of Thoracic Surgery·L Henry Edmunds

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