Truncated hantavirus nucleocapsid proteins for serotyping Sin Nombre, Andes, and Laguna Negra hantavirus infections in humans and rodents

Journal of Clinical Microbiology
Takaaki KomaJiro Arikawa

Abstract

Sin Nombre virus (SNV), Andes virus (ANDV), and Laguna Negra virus (LANV) have been known as the dominant causative agents of hantavirus pulmonary syndrome (HPS). ANDV and LANV, with different patterns of pathogenicity, exist in a sympatric relationship. Moreover, there is documented evidence of person-to-person transmission of ANDV. Therefore, it is important in clinical medicine and epidemiology to know the serotype of a hantavirus causing infection. Truncated SNV, ANDV, and LANV recombinant nucleocapsid proteins (trNs) missing 99 N-terminal amino acids (trN100) were expressed using a baculovirus system, and their applicability for serotyping SNV, ANDV, and LANV infection by the use of enzyme-linked immunosorbent assays (ELISA) was examined. HPS patient sera and natural-reservoir rodent sera infected with SNV, ANDV, and LANV showed the highest optical density (OD) values for homologous trN100 antigens. Since even patient sera with lower IgM and IgG antibody titers were serotyped, the trN100s are therefore considered useful for serotyping with early-acute-phase sera. In contrast, assays testing whole recombinant nucleocapsid protein antigens of SNV, ANDV, and LANV expressed in Escherichia coli detected homologous and heterolog...Continue Reading

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Citations

Nov 2, 2012·Epidemiology and Infection·T KomaJ Arikawa
Oct 27, 2001·Tropical Medicine & International Health : TM & IH·G CantoniE Larrieu
Aug 16, 2014·Viruses·Kumiko Yoshimatsu, Jiro Arikawa
May 9, 2016·Diagnostic Microbiology and Infectious Disease·Andrea Cristine KoishiClaudia Nunes Duarte Dos Santos
Feb 4, 2014·Virus Research·Kumiko Yoshimatsu, Jiro Arikawa
May 21, 2013·Journal of Virological Methods·Takako AmadaJiro Arikawa
Dec 27, 2019·PLoS Neglected Tropical Diseases·Bruno Tardelli Diniz NunesDaniele Barbosa de Almeida Medeiros
Dec 17, 2019·PLoS Neglected Tropical Diseases·Fernando de Paiva ConteRodrigo Nunes Rodrigues-da-Silva
Sep 8, 2018·Archives of Virology·Carolina Montoya-RuizJuan David Rodas

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