Truncated peptides from melittin and its analog with high lytic activity at endosomal pH enhance branched polyethylenimine-mediated gene transfection

The Journal of Gene Medicine
Ying-Xia TanShou-Ping Ji

Abstract

Melittin is a commonly used cell-penetrating peptide (CPP) for improving branched polyethylenimine (BPEI)-mediated gene transfection. However, its application is limited owing to the cytotoxicity generated by the lytic activity at neutral pH. In the present study, we report two truncated peptides from melittin and florae with improved transfection efficiency. Two truncated peptides consisting of 1-20 residues of melittin (MT20) and florae (FL20) were synthesized. Circular dichroism (CD) spectrometry was used to analyze the secondary structures of the peptides. The membrane-lytic activity of the peptides and their potency in enhancing cellular uptake of calcein were evaluated. The peptides and BPEI mixtures were mixed with plasmid DNA to prepare peptide/BPEI/DNA complexes. The physicochemical characters of complexes were measured and the effect of the peptides on BPEI-mediated transfection was determined. CD analysis and structure observation showed that the truncated peptides have α-helical conformation, which was necessary for penetrating activity. The truncated peptides exhibited several advantages than their parent peptides: (i) they showed higher hemolytic potency in acidic pH but lower lytic activity than their parent pept...Continue Reading

References

Jun 1, 1996·The Biochemical Journal·D E RivettJ A Werkmeister
Jul 30, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·W T GodbeyA G Mikos
Mar 4, 2000·Advanced Drug Delivery Reviews·R WattiauxM Jadot
Aug 18, 2001·Biophysical Journal·L YangH W Huang
Oct 9, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·J A WerkmeisterD E Rivett
Nov 2, 2002·The Journal of Biological Chemistry·Jean Philippe RichardBernard Lebleu
Mar 23, 2004·Pharmaceutical Research·M Laird ForrestDaniel W Pack
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Greg F WalkerErnst Wagner
May 26, 2005·Nucleic Acids Research·Martin L ReadLeonard W Seymour
Jul 30, 2005·Nature Reviews. Drug Discovery·Daniel W PackPatrick S Stayton
Mar 21, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sabine BoeckleErnst Wagner
Jul 20, 2006·Bioconjugate Chemistry·Chang-Po ChenKevin G Rice
Aug 2, 2006·Biological & Pharmaceutical Bulletin·Yuki TadaHitoshi Sasaki
Oct 20, 2006·Journal of Nanoscience and Nanotechnology·Carsten KneuerUdo Bakowsky
Jan 20, 2009·Peptides·Julien VerdonYann Héchard

❮ Previous
Next ❯

Citations

May 31, 2015·Biotechnology Advances·Kirk K HouSamuel A Wickline
Dec 25, 2012·Biomaterials·Joan G SchellingerSuzie H Pun
Sep 26, 2013·International Journal of Pharmaceutics·Hamideh ParhizMohammad Ramezani
Mar 4, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Cheng BeiXu Peisheng
Jun 14, 2018·Current Protein & Peptide Science·Can LyuBai Li
Aug 21, 2019·Current Protein & Peptide Science·Mark Dp WillcoxDebarun Dutta
Apr 30, 2015·The Journal of Pharmacology and Experimental Therapeutics·Julia DurzyńskaAnna Goździcka-Józefiak
Aug 29, 2013·The Journal of Biological Chemistry·Jing HeWilliam C Wimley
Oct 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·T Y CheangS M Wang
Jan 16, 2021·Drug Discovery Today·Bilal Ahamad ParayMohammad Z Ahmed
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vasso ApostolopoulosIstvan Toth
Mar 7, 2021·Toxins·Gandhi Rádis-Baptista

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.