Truncation of Sp1 transcription factor by myeloblastin in undifferentiated HL60 cells

Journal of Cellular Physiology
J RaoG P Studzinski

Abstract

When HL60 cells are exposed to 1,25-dihydroxyvitamin D3 (1,25D3), they undergo changes approximating the phenotype of the monocyte. Little is known, however, about the regulation and the mechanisms of this transition. It was previously noted that DNA binding by the Sp1 transcription factor in nuclear extracts of HL60 cells is profoundly altered when these cells are induced to differentiate by 1,25D3. In the present study, we show that in untreated HL60 cells only a truncated, approximately 30-kDa Sp1 fragment, encompassing the C-terminal region, binds to the GC element-containing DNA. Full-length 105-kDa Sp1 protein cannot be detected in these cells, although reverse transriptase-polymerase chain reaction reveals the presence of both 5' and 3' ends of Sp1 mRNA. Following treatment with 10(7) M 1,25D3 for 96 hr or in cells made resistant to 1,25D3 or to 1-beta-D-arabinocytosine, the Sp1 protein can be demonstrated. After an exposure to purified myeloblastin, a serine protease, purified recombinant Sp1 protein and extracts of 1,25D3-treated cells show a pattern of DNA binding similar to the pattern seen using extracts of untreated HL60 cells, indicating that the Sp1 protein is a target for myeloblastin. Because myeloblastin is pr...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J T Kadonaga, R Tjian
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D W MeltonC T Caskey
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T R BreitmanS J Collins
Jul 1, 1993·Journal of Cellular Physiology·S S Kolla, G P Studzinski

❮ Previous
Next ❯

Citations

Sep 5, 2002·Biochemical and Biophysical Research Communications·Ki-Hwan LeeMyung-Ju Ahn
Mar 28, 2003·Biochemical and Biophysical Research Communications·David JacobsonJohn P Adelman
Sep 29, 2000·Experimental Hematology·Y Shav-TalD Zipori
Oct 2, 2002·Molecular and Cellular Endocrinology·Peter Bouwman, Sjaak Philipsen
Sep 2, 2003·Cytokine & Growth Factor Reviews·Hiroshi NakajimaItsuo Iwamoto
Nov 19, 2011·The Journal of Biological Chemistry·Mauricio Di FulvioJulian Gomez-Cambronero
Dec 17, 2003·Kidney International·William F PendergraftGloria A Preston
Apr 15, 2004·Biochemical and Biophysical Research Communications·Keon MenziesMark B Taubman
Sep 19, 2003·Biochemical and Biophysical Research Communications·Kotaro SuzukiItsuo Iwamoto
Mar 30, 1999·Experimental Cell Research·F D Coffman, G P Studzinski
Aug 25, 2006·International Journal of Cancer. Journal International Du Cancer·Christine DesmedtChristos Sotiriou
Feb 6, 2004·The Journal of Biological Chemistry·Kevin L AbramsMelissa B Rogers
Nov 23, 2000·European Journal of Cell Biology·A M HussainC F Chang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.