PMID: 7524033Oct 11, 1994Paper

Trypanosoma brucei mitochondria contain RNA helicase activity

Nucleic Acids Research
A Missel, H Ulrich Göringer

Abstract

Mitochondrial gene expression in kinetoplastid organisms such as Trypanosoma, Leishmania and Crithidia requires a posttranscriptional RNA processing event known as kRNA editing. During editing, uridine nucleotides get inserted and deleted into pre-mRNAs directed by small, metabolically stable RNAs, termed guide RNAs. Although the precise mechanism of the reaction is not understood, the accepted working model describes the formation of extended anti-parallel RNA helices between gRNA molecules with pre- and partially edited mRNAs as intermediates. These duplex structures must be separated to ensure the sequential action of multiple gRNAs in a 3' to 5' polarity on the mRNA molecule. In spite of this fact, no unwinding activity has heretofore been identified in kinetoplastid mitochondria. We report the characterisation of a RNA helicase activity within Trypanosoma brucei mitochondrial extracts. The activity unwinds 25- and 48 bp, tailed RNA duplex structures but fails to separate DNA strands. It can be destroyed by heat denaturation as well as by proteinase K treatment. The activity requires magnesium cations and acts in a NTP/dNTP dependent manner. Hydrolysis of a nucleoside triphosphate is required rather than mere NTP binding as...Continue Reading

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Citations

Oct 1, 1995·Parasitology Today·S D Seiwert
Nov 18, 2000·Progress in Lipid Research·D C DrummondJ Leroux
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Feng LiLarry Simpson
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Nov 7, 2003·Nucleic Acids Research·Elizabeth A WortheyReza Salavati
Feb 5, 2009·Nucleic Acids Research·Moritz NiemannH Ulrich Göringer
Sep 22, 2012·Annual Review of Microbiology·H Ulrich Göringer
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Nov 3, 2015·Wiley Interdisciplinary Reviews. RNA·Laurie K ReadHassan Hashimi
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