Trypanosoma brucei RRM1 is a nuclear RNA-binding protein and modulator of chromatin structure

MBio
Arunasalam NaguleswaranIsabel Roditi

Abstract

TbRRM1 of Trypanosoma brucei is a nucleoprotein that was previously identified in a search for splicing factors in T. brucei. We show that TbRRM1 associates with mRNAs and with the auxiliary splicing factor polypyrimidine tract-binding protein 2, but not with components of the core spliceosome. TbRRM1 also interacts with several retrotransposon hot spot (RHS) proteins and histones. RNA immunoprecipitation of a tagged form of TbRRM1 from procyclic (insect) form trypanosomes identified ca. 1,500 transcripts that were enriched and 3,000 transcripts that were underrepresented compared to cellular mRNA. Enriched transcripts encoded RNA-binding proteins, including TbRRM1 itself, several RHS transcripts, mRNAs with long coding regions, and a high proportion of stage-regulated mRNAs that are more highly expressed in bloodstream forms. Transcripts encoding ribosomal proteins, other factors involved in translation, and procyclic-specific transcripts were underrepresented. Knockdown of TbRRM1 by RNA interference caused widespread changes in mRNA abundance, but these changes did not correlate with the binding of the protein to transcripts, and most splice sites were unchanged, negating a general role for TbRRM1 in splice site selection. Wh...Continue Reading

References

Jun 1, 1993·Molecular and Biochemical Parasitology·R Pellé, N B Murphy
Aug 28, 2001·Molecular and Biochemical Parasitology·I D Manger, J C Boothroyd
Mar 5, 2003·Molecular and Biochemical Parasitology·Christian FlückIsabel Roditi
Jan 1, 1994·Parasitology Today·P OverathK Ziegelbauer
Nov 4, 2004·Journal of Cell Science·Joanna E Lowell, George A M Cross
Nov 9, 2005·PLoS Pathogens·Simon UrwylerIsabel Roditi
Dec 13, 2005·Eukaryotic Cell·Javier De GaudenziChristine Clayton
Dec 13, 2005·Nature Structural & Molecular Biology·Eric BatschéChristian Muchardt
Aug 26, 2006·Molecular and Biochemical Parasitology·Simon HaenniIsabel Roditi
Jan 11, 2007·The Journal of Cell Biology·David Landeira, Miguel Navarro
Jun 16, 2007·Frontiers in Bioscience : a Journal and Virtual Library·John Arne Dahl, Philippe Collas
Jul 22, 2008·Nature Structural & Molecular Biology·Shengrong LinXiang-Dong Fu
Oct 9, 2008·Molecular and Biochemical Parasitology·Martin WurstChristine Clayton
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
May 12, 2009·Eukaryotic Cell·Daniela Luz AmbrósioArthur Günzl
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Nov 17, 2009·Eukaryotic Cell·Tara M Stanne, Gloria Rudenko
Sep 25, 2010·Biochemical Society Transactions·Ross Alexander, Jean D Beggs

❮ Previous
Next ❯

Citations

May 1, 2016·Current Opinion in Microbiology·Isabel RoditiArunasalam Naguleswaran
May 14, 2016·Current Opinion in Microbiology·C E Clayton
Mar 17, 2016·World Journal of Biological Chemistry·Lysangela Ronalte Alves, Samuel Goldenberg
Jul 10, 2018·The Journal of Eukaryotic Microbiology·Helisa H WippelLysangela R Alves
Sep 20, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David SchädeliPeter Bütikofer
Feb 8, 2019·The Journal of Eukaryotic Microbiology·Carolina P BañuelosDaniel O Sánchez
Jan 11, 2017·Molecular and Biochemical Parasitology·Olivera CirovicTorsten Ochsenreiter

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
RIP
reverse transcription-PCR
pulldown
RIP-Seq
PCR
transmission electron microscopy
ChIP
electrophoresis
Fluorescence microscopy

Software Mentioned

bowtie
LAS AF
TriTrypDB
7000 System SDS

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.