Abstract
The current intraspecific nomenclature in Trypanosoma cruzi describes two major lineages, named T. cruzi I and T. cruzi II, and five sublineages within T. cruzi II, named IIa, IIb, IIc, IId and IIe. The polymorphism of minicircle hypervariable regions (mHVRs) of T. cruzi has been used in many studies for the molecular characterization of parasite populations directly from biological samples. However, the molecular bases that allow strain typing by these markers are still unclear. In this work we examined forty cloned mHVRs sequences of CL-Brener reference strain (IIe sublineage), and we found a predominant group of sequences, with 40% of frequency in this strain, with a 97% of identity among them. Out of the forty clones analyzed, we identified other less representative types, and a few unique ones. This predominant sequence is also present in different reference strains belonging to the other main T. cruzi lineages and sublineages (TcI, IIa, IIb, IIc and IId) although in a many thousand times lower frequency than in the CL-Brener strain, as shown by semiquantitative PCR. Similarly, predominant mHVR sequences previously described for TcIId strains, were clearly more frequent (many thousand times higher) in the IId reference str...Continue Reading
References
Aug 7, 1992·Cell·D A Maslov, L Simpson
Jan 1, 1988·Molecular and Biochemical Parasitology·W DegraveC M Morel
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TibayrencF J Ayala
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C MorelL Simpson
Dec 20, 1996·Molecular and Biochemical Parasitology·R P SoutoB Zingales
Apr 23, 1999·Proceedings. Biological Sciences·N J Savill, P G Higgs
Jun 1, 1999·Memórias do Instituto Oswaldo Cruz·B BastrentaS F Brenière
Nov 5, 1999·Acta Tropica·N SinghA K Rastogi
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz
May 4, 2000·The American Journal of Pathology·A R VagoS D Pena
Aug 22, 2001·International Journal for Parasitology·S BrisseM Tibayrenc
Jan 11, 2003·Clinical and Diagnostic Laboratory Immunology·Carol J HolmanHenry H Balfour
Apr 18, 2003·Memórias do Instituto Oswaldo Cruz·Rodolfo DeveraJosé Rodrigues Coura
Aug 23, 2003·Experimental Parasitology·Deila J FrancoConceição R S Machado
Sep 16, 2003·Memórias do Instituto Oswaldo Cruz·Alvaro Moncayo
Jun 8, 2004·The Journal of Infectious Diseases·Marikena G RissoM Susana Leguizamon
Jul 7, 2005·Genetics·Scott J WestenbergerNancy R Sturm
Oct 11, 2005·Current Genetics·Julius LukesAlena Zíková
Apr 13, 2006·PLoS Pathogens·Jorge M de FreitasSérgio D J Pena
May 24, 2006·The Journal of Antimicrobial Chemotherapy·Ximena CoronadoAldo Solari
May 30, 2006·Experimental Parasitology·Jenny TelleriaMichal Svoboda
Nov 15, 2006·Acta Tropica·Alexander SalazarOmar Triana-Chávez
Citations
Jan 13, 2010·The American Journal of Tropical Medicine and Hygiene·Cristina DiezIván Marcipar
Jul 15, 2015·Expert Review of Anti-infective Therapy·Louisa A MessengerCaryn Bern
Apr 18, 2013·Parasitology International·Luiza de Oliveira Ramos Pereira, Adeilton Brandão
May 9, 2012·Parasitology International·Miguel H ViccoIván Marcipar
Feb 25, 2014·Acta Tropica·M Silvina Lo PrestiPatricia A Paglini-Oliva
May 22, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Iván A BontempiCristina Diez
Mar 23, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S OrtizA Solari
Jun 28, 2019·PLoS Neglected Tropical Diseases·Fanny RusmanPatricio Diosque
Jun 18, 2021·Frontiers in Cellular and Infection Microbiology·Fanny RusmanPatricio Diosque
Jul 17, 2021·Frontiers in Cellular and Infection Microbiology·Francisco Callejas-HernándezNúria Gironès
Dec 6, 2021·The Journal of Antimicrobial Chemotherapy·Juan Carlos RamírezAlejandro Gabriel Schijman