Trypanosoma cruzi: an analysis of the minicircle hypervariable regions diversity and its influence on strain typing

Experimental Parasitology
M VelazquezI S Marcipar

Abstract

The current intraspecific nomenclature in Trypanosoma cruzi describes two major lineages, named T. cruzi I and T. cruzi II, and five sublineages within T. cruzi II, named IIa, IIb, IIc, IId and IIe. The polymorphism of minicircle hypervariable regions (mHVRs) of T. cruzi has been used in many studies for the molecular characterization of parasite populations directly from biological samples. However, the molecular bases that allow strain typing by these markers are still unclear. In this work we examined forty cloned mHVRs sequences of CL-Brener reference strain (IIe sublineage), and we found a predominant group of sequences, with 40% of frequency in this strain, with a 97% of identity among them. Out of the forty clones analyzed, we identified other less representative types, and a few unique ones. This predominant sequence is also present in different reference strains belonging to the other main T. cruzi lineages and sublineages (TcI, IIa, IIb, IIc and IId) although in a many thousand times lower frequency than in the CL-Brener strain, as shown by semiquantitative PCR. Similarly, predominant mHVR sequences previously described for TcIId strains, were clearly more frequent (many thousand times higher) in the IId reference str...Continue Reading

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Citations

Jul 15, 2015·Expert Review of Anti-infective Therapy·Louisa A MessengerCaryn Bern
Apr 18, 2013·Parasitology International·Luiza de Oliveira Ramos Pereira, Adeilton Brandão
May 22, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Iván A BontempiCristina Diez
Mar 23, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S OrtizA Solari
Jun 18, 2021·Frontiers in Cellular and Infection Microbiology·Fanny RusmanPatricio Diosque
Jul 17, 2021·Frontiers in Cellular and Infection Microbiology·Francisco Callejas-HernándezNúria Gironès
Dec 6, 2021·The Journal of Antimicrobial Chemotherapy·Juan Carlos RamírezAlejandro Gabriel Schijman

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