Trypanosoma cruzi bromodomain factor 2 (BDF2) binds to acetylated histones and is accumulated after UV irradiation

International Journal for Parasitology
Gabriela Vanina VillanovaEsteban Serra

Abstract

Histone tail post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination and ADP-ribosylation) regulate many cellular processes. Among these modifications, phosphorylation, methylation and acetylation have already been described in trypanosomatid histones. Bromodomains, together with chromodomains and histone-binding SANT domains, were proposed to be responsible for "histone code" reading. The Trypanosoma cruzi genome encodes four coding sequences (CDSs) that contain a bromodomain, named TcBDF1-4. Here we show that one of those, TcBDF2, is expressed in discrete regions inside the nucleus of all the parasite life cycle stages and binds H4 and H2A purified histones from T. cruzi. Immunolocalization experiments using both anti-histone H4 acetylated peptides and anti-TcBDF2 antibodies determined that TcBDF2 co-localizes with histone H4 acetylated at lysines K10 and K14. TcDBF2 and K10 acetylated H4 interaction was confirmed by co-immunoprecipitation. It is also shown that TcBDF2 was accumulated after UV irradiation of T. cruzi epimastigotes. These results suggest that TcBDF2 could be taking part in a chromatin remodelling complex in T. cruzi.

References

Jan 1, 1988·Memórias do Instituto Oswaldo Cruz·V T ContrerasS Goldenberg
Jan 1, 1988·Experimental Cell Research·G C Toro, N Galanti
Jan 25, 1994·Nucleic Acids Research·T H Thatcher, M A Gorovsky
Oct 6, 1998·Annual Review of Biochemistry·J L Workman, R E Kingston
Jan 19, 2000·Nature·B D Strahl, C D Allis
Dec 16, 2000·Trends in Biochemical Sciences·J Wu, M Grunstein
Jan 19, 2002·EMBO Reports·Catherine M Green, Geneviève Almouzni
Jan 30, 2002·Molecular and Biochemical Parasitology·Rafael Marques PortoSergio Schenkman
Mar 26, 2002·FEBS Letters·Lei Zeng, Ming Ming Zhou
Jan 24, 2003·Nature·Gary Felsenfeld, Mark Groudine
Jul 9, 2004·Cellular Microbiology·Renata R TonelliMaria Júlia M Alves
Nov 4, 2004·Journal of Cell Science·Joanna E Lowell, George A M Cross
Jan 25, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Xavier de la CruzMarian A Martínez-Balbás
Nov 24, 2005·Journal of Cell Science·Joanna E LowellGeorge A M Cross
Apr 4, 2006·FEBS Letters·Christian J JanzenGeorge A M Cross
Oct 3, 2006·Molecular and Biochemical Parasitology·Julia Pinheiro Chagas da CunhaSergio Schenkman
Oct 10, 2006·Cellular Microbiology·William J SullivanSergio O Angel
Oct 13, 2006·Eukaryotic Cell·Mark ChandyJerry L Workman
Oct 17, 2006·Parasitology Today·N GalantiG C Toro
Oct 20, 2006·The Biochemical Journal·Ahmed H HassanTahir A Rizvi
Jan 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Thanat ChookajornKirk W Deitsch
Apr 14, 2007·The EMBO Journal·Katie HughesGloria Rudenko
Aug 19, 2007·Cell Cycle·Sophie E Polo, Geneviève Almouzni
Feb 12, 2008·Biochemical and Biophysical Research Communications·Veena MandavaGeorge A M Cross
Apr 11, 2008·The Journal of Biological Chemistry·Patricia RespuelaLena Aslund
Jul 4, 2008·PLoS Biology·Luisa M FigueiredoGeorge A M Cross

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Citations

Feb 20, 2010·Journal of Biomedicine & Biotechnology·Santiago Martínez-CalvilloElisa E Figueroa-Angulo
Oct 25, 2012·Journal of Biomedicine & Biotechnology·Victoria Lucia Alonso, Esteban Carlos Serra
Feb 15, 2013·PLoS Neglected Tropical Diseases·María de los Milagros CámaraClaudio A Pereira
Oct 15, 2009·Future Microbiology·Maria Carolina EliasSergio Schenkman
Jan 23, 2013·Cellular Microbiology·Nilmar Silvio Moretti, Sergio Schenkman
Jan 13, 2017·Microbiology and Molecular Biology Reviews : MMBR·Victoria JeffersWilliam J Sullivan
Oct 14, 2017·The Biochemical Journal·Xiao YangXiaoming Tu
May 20, 2020·Memórias do Instituto Oswaldo Cruz·Yirys Díaz-OlmosFabricio K Marchini
Jul 23, 2020·Memórias do Instituto Oswaldo Cruz·Chantal ReigadaMariana R Miranda
Nov 1, 2018·Current Medicinal Chemistry·Victoria Lucia AlonsoEsteban Serra
Nov 19, 2020·ACS Infectious Diseases·Corentine M C LaurinStuart J Conway
Feb 27, 2018·ACS Combinatorial Science·I Ayelen RamalloRicardo L E Furlan
Oct 23, 2018·ACS Medicinal Chemistry Letters·Paula GarcíaRicardo L E Furlan
Oct 7, 2021·ACS Infectious Diseases·Cynthia TallantFélix Calderón

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