Trypanosoma cruzi: identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1

Experimental Parasitology
Gisele F A PicchiStenio Perdigão Fragoso

Abstract

The nuclear lamina is a structure that lines the inner nuclear membrane. In metazoans, lamins are the primary structural components of the nuclear lamina and are involved in several processes. Eukaryotes that lack lamins have distinct proteins with homologous functions. Some years ago, a coiled-coil protein in Trypanosoma brucei, NUP-1, was identified as the major filamentous component of its nuclear lamina. However, its precise role has not been determined. We characterized a homologous protein in Trypanosoma cruzi, TcNUP-1, and identified its in vivo DNA binding sites using a chromatin immunoprecipitation assay. We demonstrate for the first time that TcNUP-1 associates with chromosomal regions containing large non-tandem arrays of genes encoding surface proteins. We therefore suggest that TcNUP-1 is a structural protein that plays an essential role in nuclear organization by anchoring T. cruzi chromosomes to the nuclear envelope.

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R P Aaronson, G Blobel
Oct 1, 1992·Molecular and Biochemical Parasitology·S P Fragoso, S Goldenberg
Aug 1, 1989·Analytical Biochemistry·J KarlinseyT Enver
Apr 1, 1988·The Journal of Cell Biology·M C BonaldoS Goldenberg
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·D M EngelmanA Goldman
Feb 13, 2001·Trends in Cell Biology·P BurkhardS V Strelkov
May 25, 1955·The Journal of Biophysical and Biochemical Cytology·M L WATSON
Oct 7, 2003·Annual Review of Biochemistry·David L Spector
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Dec 1, 2004·Annual Review of Genetics·Angela TaddeiSusan M Gasser
Jul 16, 2005·Science·Najib M El-SayedNeil Hall
Sep 17, 2005·International Review of Cytology·Howard J Worman, Jean-Claude Courvalin
Jan 4, 2006·Cellular and Molecular Life Sciences : CMLS·M A D'Angelo, M W Hetzer
Mar 24, 2006·FEMS Microbiology Letters·Edio Elígio LourençoStenio Perdigão Fragoso
Sep 13, 2008·Annual Review of Microbiology·Artur ScherfLoïc Riviere
Jun 3, 2009·BMC Genomics·D Brent WeatherlyRick L Tarleton
Aug 6, 2009·Journal of Virological Methods·Giovanny A C A MazzarottoClaudia N Duarte dos Santos

❮ Previous
Next ❯

Citations

Nov 18, 2011·Molecular Biology of the Cell·Anne KrügerRalph Gräf
Jun 21, 2014·BMC Cell Biology·Ligia Cristina KalbMaurilio José Soares
Mar 11, 2016·Current Opinion in Cell Biology·Masaaki IwamotoTokuko Haraguchi
Oct 21, 2016·PloS One·Camila OliveiraSamuel Goldenberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.