Trypanosoma cruzi: infection of cultured human endothelial cells alters inositol phosphate synthesis

Experimental Parasitology
S A MorrisMurray Wittner

Abstract

Infection of cultured endothelial cells with Trypanosoma cruzi alters intracellular Ca2+ homeostasis. To help understand the biochemical basis for this phenomenon, we determined the influence of infection on inositol phosphate formation in a broken cell preparation. Inositol phosphates participate in the regulation of cytosolic Ca2+. In uninfected endothelial cells, bradykinin guanosine 5'-O-thiophosphate (GTP tau S), and calcium all stimulated inositol phosphate (IP1), inositol bisphosphate (IP2), and inositol trisphosphate (IP3) formation within 5 sec of incubation. At longer periods of incubation with GTP tau S and bradykinin, formation of IP1 was linear for 30 sec, whereas the rate of IP2 and IP3 generation was maximal at 20 and 5 sec, respectively. Second, infection markedly changed these aspects of inositol phosphate generation. First, unstimulated (basal) levels of IP1 and IP3 were markedly increased over those levels in membranes of uninfected cells. Infection decreased the rate of formation for the three inositol phosphates in response to GTP tau S and bradykinin. Finally, infection diminished the magnitude of inositol phosphate synthesis in response to Ca2+ for IP1, IP2, and IP3, respectively. Studies on G proteins us...Continue Reading

References

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Citations

May 1, 1995·American Heart Journal·F W TorresI F Palacios
Jan 1, 1991·Parasitology Today·S A MorrisM Wittner
Feb 1, 1996·Parasitology Today·R Docampo, S N Moreno
May 4, 2001·International Journal for Parasitology·S B PetkovaH B Tanowitz
Jul 21, 1998·Journal of the American College of Cardiology·M A MathierM J Semigran
Nov 7, 2000·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·S B PetkovaH Huang
Mar 24, 2005·Infection and Immunity·Herbert B TanowitzMasashi Yanagisawa
Jan 5, 2011·PLoS Computational Biology·Andreja JovicShuichi Takayama
May 2, 2014·Life Sciences·Brandi D FreemanMahalia S Desruisseaux
Jun 12, 1999·Endothelium : Journal of Endothelial Cell Research·H B TanowitzG J Christ
Jan 1, 1992·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·H B TanowitzM Wittner
Dec 1, 1990·Circulation·S A MorrisJ P Bilezikian
Apr 11, 2020·PLoS Neglected Tropical Diseases·Alfredo MijaresJosé R Lopez
Oct 1, 1992·Clinical Microbiology Reviews·H B TanowitzM Wittner

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