PMID: 11929690Apr 4, 2002Paper

Trypanosoma cruzi: isolation of an immunodominant peptide of TESA (Trypomastigote Excreted-Secreted Antigens) by gene cloning

Diagnostic Microbiology and Infectious Disease
Tokiko K MatsumotoE S Umezawa

Abstract

Trypomastigote forms of Trypanosoma cruzi excrete-secrete several molecules, which are immunodominant during the human infection. This complex antigenic mixture termed TESA (Trypomastigote Excreted-Secreted Antigens) presents a 150-160 kDa band that shows excellent specificity and sensitivity in Chagas' disease diagnosis by immunoblotting. Here we describe the isolation and the antigenic characterization of a recombinant peptide (TESA-1) containing a 10 kDa T. cruzi peptide that belongs to the 150-160 kDa TESA fraction. The clone was isolated by screening a T. cruzi genomic expression library with chagasic antibodies reactive to the 150-160 kDa band of TESA immunoblots. After expression, the recombinant peptide TESA-1 was purified and used to immunize rabbits. Anti-TESA-1 immunesera specifically recognized the 150-160 kDa fraction of TESA-blots from eight different T. cruzi strains. The TESA-1 peptide reacted with 82.2% of chagasic patient sera by immunoblotting, showing that it harbors most of the antigenic epitopes that account for the high reactivity of the 150-160 kDa band of TESA.

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Citations

Oct 23, 2009·Journal of Clinical Microbiology·Juan David RamírezSilvia Restrepo
Feb 4, 2006·Journal of Clinical Microbiology·Mariolga BerrizbeitiaBrian J Ward
Jul 12, 2007·Kinetoplastid Biology and Disease·Francisco J AzuajeJose F Da Silveira
Feb 23, 2013·TheScientificWorldJournal·Paulo Roberto Ceridorio CorreaJosé Franco da Silveira
Sep 13, 2008·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Vicente Amato NetoCélia Regina Furucho
Jun 18, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S T BeckA W Ferreira
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Yara M GomesAlejandro O Luquetti
Nov 23, 2016·Frontiers in Chemistry·Rayner M L QueirozSébastien Charneau
Jun 29, 2017·Journal of Parasitology Research·Ángel de la Cruz Pech-CanulRosa-Lidia Solís-Oviedo
Jul 3, 2021·Life·Oscar Hernán Rodríguez-BejaranoManuel Alfonso Patarroyo

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