Trypanosoma cruzi necrotizing meningoencephalitis in a Venezuelan HIV⁺-AIDS patient: pathological diagnosis confirmed by PCR using formalin-fixed- and paraffin-embedded-tissues

Analytical Cellular Pathology (Amsterdam)
Marcello Salvatore Rossi SpadaforaMaría Flores-Chávez

Abstract

Coinfections with human immunodeficiency virus (HIV) and infectious agents have been recognized since the early 90s. In the central nervous system (CNS) of HIV(+) patients, parasitic protozoans like Toxoplasma gondii have been described as responsible for the space occupying lesions (SOL) developed. However, the involvement of Trypanosoma cruzi is also described but appears to be less frequent in acquired immunodeficiency syndrome (AIDS) and transplant recipients, associated with necrotizing myocarditis and neurological symptoms related to the occurrence of necrotizing pseudotumoral encephalitis (NPE) and meningoencephalitis (NME). The present work aims to present a Venezuelan case of NME associated with the coinfection of HIV and a T. cruzi-like trypanosomatid as well as its evolution and diagnosis by histopathological techniques, electron microscopy, and PCR methods using formalin-fixed- (FF-) and paraffin-embedded- (PE-) tissues. Postmortem cytological studies of leptomeninges imprints reveal the presence of trypomastigotes of Trypanosoma sp. Histopathological and electron microscopy studies allowed us to identify an amastigote stage and to reject the involvement of other opportunistic microorganisms as the etiological agent...Continue Reading

References

Oct 1, 1992·Clinical Microbiology Reviews·H B TanowitzM Wittner
Dec 1, 1991·The American Journal of Tropical Medicine and Hygiene·M S FerreiraS Tostes Júnior
Jul 1, 1989·Journal of Clinical Microbiology·D R MoserJ E Donelson
Mar 1, 1994·The American Journal of Tropical Medicine and Hygiene·A RochaE R Lopes
Nov 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A M SartoriM Amato Shikanai-Yasuda
Oct 1, 1996·Journal of Clinical Microbiology·I FuentesJ Alvar
Dec 1, 1996·Journal of Clinical Microbiology·J N VelásquezM I Rodríguez
Oct 10, 1997·Revista Da Sociedade Brasileira De Medicina Tropical·A G GuevaraR H Guderian
Aug 24, 1999·Nucleic Acids Research·N J CoombsJ N Primrose
Aug 27, 1999·The American Journal of Tropical Medicine and Hygiene·L Perez-RamirezM Tibayrenc
Jan 13, 2001·AIDS Patient Care and STDs·M Corti
May 15, 2004·Kinetoplastid Biology and Disease·Anil K VaidianHerbert B Tanowitz
Mar 7, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kiyohiro HamataniKei Nakachi
Jul 26, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marshall S ScicchitanoRogely W Boyce
Feb 6, 2007·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Samih H NasrVivette D D'Agati
Dec 6, 2007·Memórias do Instituto Oswaldo Cruz·José Rodrigues Coura
Mar 14, 2008·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Ezequiel CordovaMarcelo Corti
Apr 28, 2009·The Lancet Infectious Diseases·Carlos A DiazgranadosCarlos Franco-Paredes
Jul 30, 2009·Transplant Infectious Disease : an Official Journal of the Transplantation Society·J A DavisJ A Fishman
Apr 21, 2010·The Medical Journal of Australia·Samuel C HumePeter A Stanley
Oct 12, 2010·Clinical Microbiology Reviews·J L N BarrattD Stark
Jan 1, 2011·Tropical Parasitology·Rohit Chawla, R L Ichhpujani
Dec 18, 2013·Indian Journal of Sexually Transmitted Diseases and AIDS·Ajay MalikVibha Dutta
Mar 22, 2014·Biomédica : revista del Instituto Nacional de Salud·Zulbey Rivero-RodríguezRafael Villalobos

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Citations

Jun 19, 2019·Emerging Infectious Diseases·Ashrit MultaniBrian G Blackburn

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Methods Mentioned

BETA
transmission
PCR
transmission electron microscopy
electrophoresis
ELISA

Related Concepts

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