Trypanosoma evansi: genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates

Experimental Parasitology
Daniel K MasigaC Michael R Turner

Abstract

We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses. AFLP accessed many more polymorphisms than RAPD. Cluster analysis of the AFLP data showed that 12 T.evansi isolates were very similar ('type A') whereas 2 isolates differed substantially ('type B'). Type A isolates have been generally regarded as genetically identical but AFLP analysis was able to identify multiple differences between them and split the type A T. evansi isolates into two distinct clades.

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Citations

Jun 26, 2007·Parasitology Research·Z K Njiru, C C Constantine
Sep 26, 2012·Parasitology Research·Mehrdad PourjafarNarges Hosseini Joshani-Zadeh
Jun 11, 2016·Veterinary World·Biswa Ranjan MaharanaNaduvanahalli Rajanna Sudhakar
Dec 14, 2011·PloS One·Paweł MilczarskiMonika Rakoczy-Trojanowska
May 17, 2006·Trends in Ecology & Evolution·Richard A EnnosPeter M Hollingsworth
Sep 8, 2017·PLoS Neglected Tropical Diseases·Christine M KamidiAdalgisa Caccone
Apr 2, 2016·PLoS Neglected Tropical Diseases·Hadush BirhanuNick Van Reet
Aug 9, 2020·Veterinary Sciences·Ellen Clare Hughes, Neil Euan Anderson
Apr 2, 2019·Veterinary Parasitology : Regional Studies and Reports·Rajender KumarB N Tripathi

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