PMID: 8383313Feb 1, 1993Paper

Trypanosoma (Nannomonas) congolense: molecular characterization of a new genotype from Tsavo, Kenya

Parasitology
P A MajiwaE Zweygarth

Abstract

Trypanosoma (Nannomonas) congolense comprises morphologically identical but genetically heterogeneous parasites infective to livestock and other mammalian hosts; three different genotypes of this parasite have been described previously. Restriction enzyme fragment length polymorphisms (RFLPs) in both kinetoplast DNA minicircle and nuclear DNA sequences, and randomly amplified polymorphic deoxyribonucleic acid (RAPD) patterns have been used here to demonstrate the existence of another type of T. (N.) congolense that is genotypically distinct from those that have so far been characterized at the molecular level. A highly repetitive, tandemly arranged DNA sequence and oligonucleotide primers, for use in polymerase chain reaction (PCR) amplification are described, which can be used for specific identification of the trypanosome and its distinction from others within the Nannomonas subgenus.

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Citations

Mar 1, 1996·Veterinary Parasitology·D K MasigaW C Gibson
Mar 17, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Z K NjiruW C Gibson
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Wendy Gibson
Mar 4, 2000·Molecular and Biochemical Parasitology·K AsbeckW Gibson
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Aug 23, 2001·Veterinary Parasitology·R Prichard, A Tait
Jul 25, 2003·Veterinary Parasitology·Serap Aksoy
Nov 26, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Imna MaleleWendy Gibson
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Oct 30, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·P B HamiltonW C Gibson
May 8, 2009·The Journal of Veterinary Medical Science·Zhang JingNoboru Inoue
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Apr 25, 2007·International Journal for Parasitology·W Gibson
Mar 1, 1993·The Journal of Eukaryotic Microbiology·M F DirieP R Gardiner

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