PMID: 6689336Nov 1, 1983

Trypanosoma rhodesiense bloodstream trypomastigote and culture procyclic cell surface carbohydrates

The Journal of protozoology
P R Jackson, C L Diggs


Bloodstream trypomastigote and culture procyclic (insect midgut) forms of a cloned T. rhodesiense variant (WRAT at 1) were tested for agglutination with the lectins concanavalin A (Con A), phytohemagglutinin P (PP), soybean agglutinin (SBA), fucose binding protein (FBP), wheat germ agglutinin (WGA), and castor bean lectin (RCA). Fluorescence-microscopic localization of lectin binding to both formalin-fixed trypomastigotes and red cells was determined with fluorescein isothiocyanate (FITC)-conjugated Con A, SBA, FBP, WGA, RCA, PNA (peanut agglutinin), DBA (Dolichos bifloris), and UEA (Ulex europaeus) lectins. Electron microscopic localization of lectin binding sites on bloodstream trypomastigotes was accomplished by the Con A-horseradish peroxidase-diamino-benzidine (HRP-DAB) technique, and by a Con A-biotin/avidin-ferritin method. Trypomastigotes, isolated by centrifugation or filtration through DEAE-cellulose or thawed after cryopreservation, were agglutinated by the lectins Con A and PP with agglutination strength scored as Con A greater than PP. No agglutination was observed in control preparations or with the lectins WGA, FBA or SBA. Red cells were agglutinated by all the lectins tested. Formalin-fixed bloodstream trypomast...Continue Reading


Feb 1, 1979·The Journal of protozoology·K L Banks
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A F Barbet, T C McGuire
Nov 1, 1979·The American Journal of Tropical Medicine and Hygiene·G H CampbellC L Diggs
Mar 15, 1979·The Biochemical Journal·J G Johnson, G A Cross
May 1, 1978·The Journal of protozoology·K L Banks
Jan 1, 1973·Annual Review of Biochemistry·H Lis, N Sharon
Dec 1, 1970·Experimental Parasitology·S M Lanham, D G Godfrey
Sep 15, 1972·Science·N Sharon, H Lis
Jan 1, 1982·Annual Review of Biochemistry·P T EnglundJ C Marini
Sep 1, 1980·The American Journal of Tropical Medicine and Hygiene·G A CrossJ C Boothroyd
Aug 1, 1980·Immunology Today·D M Weir

Related Concepts

Fucose-mannose ligand
Soybean lectin
Agglutination Tests
Plasma Membrane
Concanavalin A
Receptors, Concanavalin A
Receptors, Mitogen
Ricin I

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.