PMID: 6165395May 29, 1981Paper

Trypsin activation of enterotoxin from Clostridium perfringens type A: fragmentation and some physicochemical properties

Biochimica Et Biophysica Acta
P E GranumR Skjelkvåle

Abstract

Clostridium perfringens type A enterotoxin was activated about 3-fold by treatment with trypsin, without an observed change in molecular weight. On denaturation in 8 M urea, the trypsinated enterotoxin lost a small peptide of about 4000 daltons. The single cysteine residue of enterotoxin was in the small peptide together with seven out of nine residues of proline. Trypsin activation, without removal of the small peptide, increased the 'outside' number of amino groups from eight to eleven. The trypsin treatment of the enterotoxin did not change the antigenic properties of the protein. Glycine was the C-terminal residue of the native enterotoxin while the dansyl alpha-amino acid of the N-terminal could not be identified.

References

Aug 1, 1977·Infection and Immunity·I Ohishi, G Sakaguchi
Jul 1, 1979·Canadian Journal of Biochemistry·P E Penner
Jul 15, 1978·Analytical Biochemistry·M R Sutton, R A Bradshaw
Nov 1, 1972·Infection and Immunity·R L Stark, C L Duncan
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Jun 1, 1980·Applied and Environmental Microbiology·P E Granum, J R Whitaker
Oct 1, 1980·Analytical Biochemistry·J R WhitakerG Aasen
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Jan 1, 1972·Methods in Enzymology·R Fields
Feb 1, 1978·Journal of Food Protection·H S Naik, C L Duncan

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Citations

Jan 1, 1984·The International Journal of Biochemistry·V P NoskovaA N Noskov
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·P E Granum, M Richardson
Oct 31, 1986·Biochimica Et Biophysica Acta·Y HoriguchiG Sakaguchi
Mar 1, 1990·International Journal of Food Microbiology·P E Granum
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·B A McClane
May 2, 2002·International Journal of Food Microbiology·Sigrid Brynestad, Per Einar Granum
Mar 25, 1985·FEBS Letters·M Richardson, P E Granum
Jan 1, 1997·The Journal of Veterinary Medical Science·K NagataT Uemura
Jun 1, 1986·The Journal of Applied Bacteriology·D ReynoldsP Hambleton
Feb 1, 2000·The Journal of Biological Chemistry·J F Kokai-KunA D O'Brien
Apr 24, 2021·Tropical Animal Health and Production·Lida Abdolmohammadi Khiav, Azadeh Zahmatkesh
Jun 1, 1990·Infection and Immunity·K B Park, R G Labbé
Jan 1, 1990·Clinical Microbiology Reviews·C L Hatheway
Jul 1, 1985·Journal of Bacteriology·S P Howard, J T Buckley
May 1, 1992·Infection and Immunity·P C HannaB A McClane
Sep 22, 2018·Microbiology Spectrum·Archana ShresthaBruce A McClane
Mar 1, 1997·Infection and Immunity·J F Kokai-Kun, B A McClane

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