PMID: 19275741Mar 12, 2009Paper

Trypsin-chymotrypsin inhibitors from Vigna mungo seeds

Protein and Peptide Letters
Allen H K CheungT B Ng

Abstract

Three trypsin-chymotrypsin inhibitors were isolated from seeds of the black gram (Vigna mungo) with a procedure that entailed cation exchange chromatography on SP-Sepharose, anion exchange chromatography on Q-Sepharose, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono Q and Mono S, and gel filtration by FPLC on Superdex 75. Two of the trypsin-chymotrypsin inhibitors were adsorbed on the first four types of chromatographic media. All three inhibitors have a molecular mass of 16 kDa as judged by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The trypsin inhibitory activity of the inhibitors was attenuated in the presence of the reducing agent dithiothreitol. The remaining inhibitor was unadsorbed on SP-Sepharose but adsorbed on Q-Sepharose, Mono Q and Mono S. The protease inhibitors did not exert any inhibitory effect on hepatoma (Hep G2) and breast cancer (MCF 7) cells or antifungal action toward Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola. Two of the inhibitors slightly inhibited the activity of HIV-1 reverse transcriptase, with an IC50 in the millimolar range.

Citations

Jan 12, 2013·Applied Biochemistry and Biotechnology·Yau Sang ChanTzi Bun Ng
Apr 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Xiu Juan Ye, Tzi Bun Ng
Dec 8, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·S K Lam, T B Ng
Jul 19, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Yau Sang ChanTzi Bun Ng
Apr 7, 2010·Biochimie·Maria Luiza V OlivaMisako U Sampaio
Jan 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Miao LiTzi Bun Ng
Oct 11, 2019·PloS One·Graziele Cristina FerreiraSergio Daishi Sasaki
Sep 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xixi CaiShaoyun Wang

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