PMID: 9435914Nov 1, 1994Paper

Trypsin inhibitor activities of fibroblasts increase with age of donor and are unaltered in familial Alzheimer's disease

Experimental Gerontology
M G HearnK Fukuchi

Abstract

Increasing evidence suggests that proteases and their inhibitors play an important role in the etiology of beta-amyloidogenesis and Alzheimer's disease (AD). It is not clear, however, which proteases and protease inhibitors are responsible for the amyloidogenic proteolysis. Candidates include alpha-1-antichymotrypsin, inter-alpha-trypsin inhibitor, and forms of beta-amyloid precursor protein (beta PP) bearing Kunitz protease inhibitor domains. As one approach to this question, we have determined the trypsin inhibitor activity of fibroblast-like cells from 10 familial AD subjects and 20 controls. The activity was quantitated by measuring remaining trypsin activity of reaction mixtures containing trypsin and cell lysates using a fluorogenic substrate and two physiologically distinct populations of fibroblasts: proliferating cells (grown in the presence of 16% serum) and quiescent cells (maintained in 0.1% serum). The remaining trypsin activities of crude protein extracts from proliferating and quiescent AD cultures were not significantly different from those of controls. Perhaps of more general interest to the biology of aging, however, was our finding that protease inhibitor activity increased with the age of the donor (p = 0.005).

References

Dec 1, 1992·Nature Genetics·P St George-HyslopD Crapper McLachlan
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J AdlerN N Dewji
Jan 31, 1991·Biochemical and Biophysical Research Communications·E YoshidaH Mihara
Mar 1, 1991·Mutation Research·G CarmelietJ J Cassiman
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·W E Van NostrandD D Cunningham
Jan 31, 1991·Biochemical and Biophysical Research Communications·C R AbrahamP Tempst
Feb 11, 1988·Nature·R W Carrell
Nov 1, 1989·Mechanisms of Ageing and Development·M F EthierJ G Dobson
Feb 20, 1987·Science·P H St George-HyslopD Drachman
Jan 1, 1989·Annals of Neurology·T D BirdJ Tinklenberg
Feb 1, 1985·International Journal of Peptide and Protein Research·Y Birk
Jan 1, 1986·Journal of Cellular Physiology·P M BartoldR C Page
Sep 10, 1970·Biochemical and Biophysical Research Communications·T W Wang, B Kassell
Jan 1, 1983·Annual Review of Biochemistry·J Travis, G S Salvesen
Jan 1, 1982·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·K J PerlR R Cairns
Apr 1, 1983·Archives of Neurology·L E NeeM Ebert
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D J Knauer, D D Cunningham

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Citations

Jan 1, 1995·Molecular Aspects of Medicine·S I Rattan
Jan 1, 1996·Experimental Gerontology·S I Rattan

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