Trypsin inhibitor from Poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases

The Protein Journal
Viviane Alves GarciaMaria Lígia Rodrigues Macedo

Abstract

Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves of being attacked by insects. In this work, a novel trypsin inhibitor (PPTI) was purified from the seeds of the native Brazilian tree Poecilanthe parviflora (Benth) (Papilioinodeae, Leguminosae) by gel filtration chromatography on a Sephadex G-100 followed by Superdex G75 chromatography (FPLC), Sepharose 4B-Trypsin column, and fractionated by reversed-phase HPLC on a C-18 column. SDS-PAGE showed that PPTI consisted of a single polypeptide chain with molecular mass of about 16 kDa. The dissociation constant of 1.0 x 10(-7) M was obtained with bovine trypsin. PPTI was stable over a wide range of temperature and pH and in the presence of DTT. The N-terminal sequence of the PPTI showed a high degree of homology with other Kunitz-type inhibitors. Trypsin-like activity in midguts of larval Diatraea saccharalis, Anagasta kuehniella, Spodoptera frugiperda, and Corcyra cephalonica were substantially inhibited by PPTI.

Citations

Dec 15, 2012·Plant Physiology and Biochemistry : PPB·Fabiola P S RufinoAdeliana S Oliveira
Nov 26, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Maria Lígia Rodrigues MacedoCaio Fernando Ramalho de Oliveira
Mar 11, 2010·Experimental Parasitology·Daosheng YiXiangrui Li
Nov 15, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Vanessa da Silveira RamosMaria Lígia Rodrigues Macedo
Mar 25, 2008·Biochimica Et Biophysica Acta·M H Lingaraju, Lalitha R Gowda
Mar 18, 2010·Archives of Insect Biochemistry and Physiology·Maria Lígia Rodrigues MacedoJosé Roberto Postali Parra
Jun 19, 2013·Plant Physiology and Biochemistry : PPB·Ana C B CruzElizeu A Santos
Apr 9, 2015·Acta Biochimica Et Biophysica Sinica·Junchen LyuShiming Deng
Apr 7, 2010·Biochimie·Maria Luiza V OlivaMisako U Sampaio
Mar 11, 2015·Pesticide Biochemistry and Physiology·Lays Cordeiro GuimarãesMaria Lígia Rodrigues Macedo
Aug 14, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Vanessa da S RamosMaria L R Macedo
Jan 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Miao LiTzi Bun Ng
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Feb 2, 2020·Plant Science : an International Journal of Experimental Plant Biology·Juliana CotabarrenWalter David Obregón
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Apr 11, 2021·BMC Plant Biology·Franziska EberlSybille B Unsicker
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Karina Margareti Alencar de BarrosMaria Lígia Rodrigues Macedo

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