Trypsin is a multifunctional factor in spermatogenesis.

Proceedings of the National Academy of Sciences of the United States of America
C MiuraT Miura

Abstract

Trypsin is well known as a pancreatic enzyme that is typically secreted into the intestine to digest proteins. We show in our current study, however, that trypsin is also a key factor in the control of spermatogenesis. A progestin in teleost fish, 17alpha, 20beta-dihydroxy-4-pregnen-3-one (DHP), is an essential component of the spermatogenesis pathway, particularly during the initiation of the first meiotic division. In the course of our investigations into the mechanisms underlying progestin-stimulated spermatogenesis, we identified that eel trypsinogen is upregulated in eel testis by DHP treatment. Trypsinogen is expressed in the Sertoli cells surrounding spermatogonia and in the membranes of spermatids and spermatozoa. Using an in vitro eel testicular culture system, we further analyzed the roles of trypsin in spermatogenesis. The inhibition of trypsin using specific antibodies or serine protease inhibitors was found to compromise DHP-induced spermatogenesis. A low dose of trypsin induces DNA synthesis and the expression of Spo11, a molecular marker of meiosis, in germ cells. By comparison, a higher dose of trypsin partially induced spermiogenesis. Furthermore, trypsin was detectable in the membranes of the spermatozoa and f...Continue Reading

References

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Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Katsueki Ogiwara, Takayuki Takahashi

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Citations

Nov 3, 2011·Amino Acids·Masato HiguchiTakeshi Miura
Jan 20, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Shi X ChenRüdiger W Schulz
Dec 9, 2014·Zoological Science·Sanath RajapakseTakayki Takahashi
Feb 6, 2016·Andrology·L R FrançaM D Griswold
Jan 31, 2013·General and Comparative Endocrinology·Shi X ChenRüdiger W Schulz
Oct 25, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Bu-Chin YuSue-Joan Chang
Jul 15, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Liang ChiJun Li
Apr 4, 2017·Biology of Reproduction·Ryuzo YanagimachiGary N Cherr

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