PMID: 9540855Apr 16, 1998Paper

Trypsinogen activation in rat pancreatic acinar cells hyperstimulated by caerulein

Biochimica Et Biophysica Acta
W HalangkH Lippert

Abstract

Inappropriate trypsinogen activation is discussed as an early intracellular event in the secretagogue-induced model of acute pancreatitis. However, the mechanisms by which trypsinogen is activated are not well characterized. In the present work, trypsinogen activation was studied in intact acinar cells using bis-(CBZ-arginyl)-Rhodamine 110 [(CBZ-Arg)2-Rho 110] as a cell-permeant substrate for trypsin and also independently via the formation of trypsinogen activation peptide (TAP). Preincubation with 10 nM caerulein increased the Rho 110-substrate cleavage more than threefold. This proteolytic activity was fully sensitive to a benzamidine (BA)-type serine protease inhibitor. The appearance of enzymatic activity was paralleled by the formation of TAP. The lack of effect of the high-molecular soybean trypsin inhibitor indicates an intracellular substrate cleavage. The cathepsin B inhibitor CA-074 prevented neither the caerulein-induced formation of TAP nor the (CBZ-Arg)2-Rho 110-cleaving activity. BA inhibited the Rho 110-substrate cleavage and significantly reduced the TAP formation. These results show that trypsinogen activation in caerulein-hyperstimulated acinar cells may occur independently of the activity of cathepsin B. On ...Continue Reading

References

Jul 1, 1992·Biological Chemistry Hoppe-Seyler·G RotheG Valet
Jan 1, 1989·Pancreas·H YamaguchiH Nawata
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A Amsterdam, J D Jamieson
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R T JensenJ D Gardner
Aug 1, 1995·Gastroenterology·F S Gorelick, L M Matovcik

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Citations

Feb 19, 2003·Research in Veterinary Science·Caroline S MansfieldT Spillman
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·M RaratyO H Petersen
Mar 26, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Eugene P CeppaFiore Cattaruzza
Jun 26, 2014·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Cora WexHans Lippert
Jun 27, 2019·Scientific Reports·Alexandra DemcsákMiklós Sahin-Tóth
Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Walter HalangkMarkus M Lerch
Feb 16, 2021·Journal of Veterinary Internal Medicine·Marnin A FormanKatja Steiger

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