Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry

Analytica Chimica Acta
Wim FremoutLuc Moens

Abstract

A proteomics approach was used for the identification of protein binders in historical paints: the proteins were digested enzymatically into peptides using trypsin before being separated and detected by high performance liquid chromatography-electrospray ionisation tandem mass spectrometry (HPLC-ESI-MS/MS). Mascot (Matrix Science) was used to analyse the resulting data and for protein identification. In contrast to amino acid analysis, amino acid sequences could be studied that retain much more information about the proteins. The best extraction strategy was selected based on the number of peptides that were identified in the protein content of paint replicas using different methods. The influence of pigments on the extraction method was studied and the analytical characteristics of the selected method were determined. Finally this method was applied to historical paint microsamples on the anonymous early 15th century panel painting Crucifixion with St Catherine and St Barbara (Calvary of the Tanners), the St Catherine Altarpiece by Joes Beyaert (c. 1479) and two paintings by Pieter Brueghel the Younger (1617-1628).

References

May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Masamichi Oh-Ishi, Tadakazu Maeda
Mar 14, 2003·Nature·Ruedi Aebersold, Matthias Mann
Feb 6, 2007·Journal of Chromatography. a·Benito CañasJose Manuel Gallardo
Jun 25, 2008·Analytica Chimica Acta·María Teresa Doménech-Carbó

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Citations

Oct 5, 2010·Analytical and Bioanalytical Chemistry·Sophie DallongevilleCaroline Tokarski
May 16, 2013·Journal of Biosciences·Michela GambinoFabio Forlani
Jul 31, 2012·International Journal of Legal Medicine·Katleen Van SteendamDieter Deforce
Dec 29, 2015·Chemical Reviews·Sophie DallongevilleCaroline Tokarski
Sep 25, 2015·Analytical Chemistry·Roberto VinciguerraLeila Birolo
Aug 26, 2016·Analytical and Bioanalytical Chemistry·Cosima Damiana CalvanoLuigia Sabbatini
Oct 22, 2011·Analytical Chemistry·Sophie DallongevilleCaroline Tokarski
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lukáš KučeraPetr Bednář
Feb 15, 2017·Analytical Chemistry·Marcello ManfrediPier Giorgio Righetti

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