Tryptophan autofluorescence imaging of neoplasms of the human colon

Journal of Biomedical Optics
Bhaskar BanerjeeUrs Utzinger

Abstract

Detection of flat neoplasia is a major challenge in colorectal cancer screening, as missed lesions can lead to the development of an unexpected 'incident' cancer prior to the subsequent endoscopy. The use of a tryptophan-related autofluorescence has been reported to be increased in murine intestinal dysplasia. The emission spectra of cells isolated from human adenocarcinoma and normal mucosa of the colon were studied and showed markedly greater emission intensity from cancerous cells compared to cells obtained from the surrounding normal mucosa. A proto-type multispectral imaging system optimized for ultraviolet macroscopic imaging of tissue was used to obtain autofluorescence images of surgical specimens of colonic neoplasms and normal mucosa after resection. Fluorescence images did not display the expected greater emission from the tumor as compared to the normal mucosa, most probably due to increased optical absorption and scattering in the tumors. Increased fluorescence intensity in neoplasms was observed however, once fluorescence images were corrected using reflectance images. Tryptophan fluorescence alone may be useful in differentiating normal and cancerous cells, while in tissues its autofluorescence image divided by g...Continue Reading

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Citations

Jan 8, 2013·Journal of Biomedical Optics·Timothy E RenkoskiUrs Utzinger
Oct 12, 2013·Lasers in Surgery and Medicine·Bhaskar BanerjeeUrs Utzinger
May 26, 2016·Journal of Biomedical Optics·Tyler H TateJennifer Kehlet Barton
Jan 29, 2016·Biomedical Optics Express·Yasuaki KumamotoSatoshi Kawata

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