Tryptophan decarboxylase plays an important role in ajmalicine biosynthesis in Rauvolfia verticillata.

Planta
Wanhong LiuZhihua Liao

Abstract

Tryptophan decarboxylase (TDC) converts tryptophan into tryptamine that is the indole moiety of ajmalicine. The full-length cDNA of Rauvolfia verticillata (RvTDC) was 1,772 bps that contained a 1,500-bp ORF encoding a 499-amino-acid polypeptide. Recombinant 55.5 kDa RvTDC converted tryptophan into tryptamine. The K (m) of RvTDC for tryptophan was 2.89 mM, higher than those reported in other TIAs-producing plants. It demonstrated that RvTDC had lower affinity to tryptophan than other plant TDCs. The K (m) of RvTDC was also much higher than that of strictosidine synthase and strictosidine glucosidase in Rauvolfia. This suggested that TDC might be the committed-step enzyme involved in ajmalicine biosynthesis in R. verticillata. The expression of RvTDC was slightly upregulated by MeJA; the five MEP pathway genes and SGD showed no positive response to MeJA; and STR was sharply downregulated by MeJA. MeJA-treated hairy roots produced higher level of ajmalicine (0.270 mg g(-1) DW) than the EtOH control (0.183 mg g(-1) DW). Highest RvTDC expression level was detected in hairy root, about respectively 11, 19, 65, and 109-fold higher than in bark, young leaf, old lea...Continue Reading

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Citations

Nov 19, 2013·Biotechnology and Applied Biochemistry·Kai ChangZhihua Liao
Aug 29, 2013·Plant Biotechnology Journal·Joseph G DubouzetKyo Wakasa
Oct 10, 2018·Chembiochem : a European Journal of Chemical Biology·Jakob FrankeSarah E O'Connor
Aug 4, 2020·Natural Product Reports·Benjamin R Lichman
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chuxin LiangRongmin Yu
May 20, 2021·Journal of Experimental Botany·Stefano NegriFlavia Guzzo

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