Abstract
Plasma tryptophan (Trp) depletion is a commonly used tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders. This study measured the cerebrospinal fluid (CSF) monoamine metabolite response to Trp depletion and control testing in five healthy subjects utilizing a single lumbar puncture. Testing was done in a placebo-controlled, double-blind, randomized, cross-over design. Plasma-free and total Trp levels and behavioural ratings were obtained prior to and 5 h after ingestion of each amino-acid drink. CSF was obtained by performing a standard lumbar puncture 7 h after ingestion of the drink. Compared to control testing, Trp depletion caused a significant decrease of CSF 5-hydroxyindoleacetic acid (5-HIAA) (p = 0.03), but not of homovanillic acid or 3-methoxy-4-hydroxy-phenylglycol. Behavioural ratings were minimally affected in all subjects. This confirms that plasma Trp depletion reduces central nervous system measures of 5-HT function and suggests that the single lumbar puncture technique may be sufficient to detect the extent of CSF 5-HIAA changes during Trp depletion studies.
Citations
Sep 29, 2007·European Archives of Psychiatry and Clinical Neuroscience·Christine NorraHanns Jürgen Kunert
Aug 14, 2003·Psychiatry Research·Duncan B Clark
Oct 31, 2013·Neurochemical Research·Haipeng LiuPeng Xie
Aug 25, 1999·Biological Psychiatry·F A MorenoP L Delgado
Jul 8, 2009·Neuroscience·E L van DonkelaarP A T Kelly
Jan 8, 2008·Physiology & Behavior·Anu K KoskelaAila Rissanen
Jun 20, 2003·European Journal of Clinical Investigation·B WirleitnerD Fuchs
Dec 21, 2004·Biological Psychiatry·Matthew S MilakRamin V Parsey
Sep 29, 2004·Biological Psychiatry·Audrey R TyrkaLawrence H Price
May 6, 2015·CNS Spectrums·Daniel R Rosell, Larry J Siever
Oct 30, 2016·Neuroscience and Biobehavioral Reviews·Kyeon RaabDaniela Mier
Feb 23, 2011·Molecular Psychiatry·E L van DonkelaarJ Prickaerts
May 9, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ronald M SalomonMichael H Ebert
Feb 13, 2002·Molecular Psychiatry·F A MorenoP L Delgado
Feb 4, 2012·The American Journal of Clinical Nutrition·Daniel KeszthelyiAd A Masclee
Aug 8, 2015·Pteridines·Uttam K RahejaTeodor T Postolache
Mar 7, 2020·BMC Psychiatry·Xiacan ChenJunmei Hu
Nov 6, 2020·International Journal of Tryptophan Research : IJTR·Luca Aquili
Sep 10, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Robert ZangerleDietmar Fuchs