Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: results from single lumbar puncture sampling technique

The International Journal of Neuropsychopharmacology
Francisco A. MorenoP L Delgado

Abstract

Plasma tryptophan (Trp) depletion is a commonly used tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders. This study measured the cerebrospinal fluid (CSF) monoamine metabolite response to Trp depletion and control testing in five healthy subjects utilizing a single lumbar puncture. Testing was done in a placebo-controlled, double-blind, randomized, cross-over design. Plasma-free and total Trp levels and behavioural ratings were obtained prior to and 5 h after ingestion of each amino-acid drink. CSF was obtained by performing a standard lumbar puncture 7 h after ingestion of the drink. Compared to control testing, Trp depletion caused a significant decrease of CSF 5-hydroxyindoleacetic acid (5-HIAA) (p = 0.03), but not of homovanillic acid or 3-methoxy-4-hydroxy-phenylglycol. Behavioural ratings were minimally affected in all subjects. This confirms that plasma Trp depletion reduces central nervous system measures of 5-HT function and suggests that the single lumbar puncture technique may be sufficient to detect the extent of CSF 5-HIAA changes during Trp depletion studies.

Citations

Sep 29, 2007·European Archives of Psychiatry and Clinical Neuroscience·Christine NorraHanns Jürgen Kunert
Oct 31, 2013·Neurochemical Research·Haipeng LiuPeng Xie
Aug 25, 1999·Biological Psychiatry·F A MorenoP L Delgado
Jun 20, 2003·European Journal of Clinical Investigation·B WirleitnerD Fuchs
May 6, 2015·CNS Spectrums·Daniel R Rosell, Larry J Siever
Feb 23, 2011·Molecular Psychiatry·E L van DonkelaarJ Prickaerts
May 9, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ronald M SalomonMichael H Ebert
Feb 4, 2012·The American Journal of Clinical Nutrition·Daniel KeszthelyiAd A Masclee
Nov 6, 2020·International Journal of Tryptophan Research : IJTR·Luca Aquili
Sep 10, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Robert ZangerleDietmar Fuchs

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