Tryptophan fluorescence in the Bacillus subtilis phototropin-related protein YtvA as a marker of interdomain interaction

Photochemistry and Photobiology
Aba LosiW Gärtner

Abstract

The Bacillus subtilis protein YtvA, related to plant phototropins (phot), binds flavin mononucleotide (FMN) within the N-terminal light, oxygen and voltage (LOV) domain. The blue light-triggered photocycle of YtvA and phot involves the reversible formation of a covalent photoadduct between FMN and a cysteine (cys) residue. YtvA contains a single tryptophan, W103, localized on the LOV domain and conserved in all phot-LOV domains. In this study, we show that the fluorescence parameters of W103 in YtvA-LOV are markedly different from those observed in the full-length YtvA. The fluorescence quantum yields are ca 0.03 and 0.08, respectively. In YtvA-LOV, the maximum is redshifted (ca 345 vs 335 nm) and the average fluorescence lifetime shorter (2.7 vs 4.7 ns). These data indicate that W103 is located in a site of tight contact between the two domains of YtvA. In the FMN-cys adduct, selective excitation of W103 at 295 nm results in minimal changes of the fluorescence parameters with respect to the dark state. On 280 nm excitation, however, there is a detectable decrease in the fluorescence emitted from tyrosines, with concomitant increase in W103 fluorescence. This effect is reversible in the dark and might arise from a light-regulat...Continue Reading

References

Jul 17, 1998·Biochemistry·Y Chen, M D Barkley
Feb 9, 2000·Current Biology : CB·L Aravind, E V Koonin
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Crosson, K Moffat
Jun 27, 2001·The Plant Cell·W R BriggsJ C Watson
Apr 20, 2002·Biophysical Journal·Aba LosiWolfgang Gärtner
Apr 12, 2003·Biochemistry·Michael SalomonWolfhart Rüdiger
Aug 13, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Aba LosiWolfgang Gärtner
Sep 13, 2003·Science·Shannon M HarperKevin H Gardner

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Citations

Apr 20, 2007·European Biophysics Journal : EBJ·Valentina ButtaniAba Losi
Jul 8, 2009·The Journal of Biological Chemistry·Marcela Avila-PérezKlaas Hellingwerf
Aug 23, 2006·Journal of Bacteriology·Tatiana A GaidenkoChester W Price
Sep 4, 2007·Journal of Molecular Biology·Andreas Möglich, Keith Moffat
Mar 13, 2012·Journal of Molecular Biology·Josiah P ZaynerTobin R Sosnick
Jul 9, 2016·Journal of Photochemistry and Photobiology. B, Biology·Parul PariharSheo Mohan Prasad
Jun 19, 2007·The Journal of General and Applied Microbiology·Noriko SuzukiAkira Nakamura
Jan 12, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yifen TangAba Losi

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Methods Mentioned

BETA
infrared spectroscopy
fluorescence spectroscopy
nuclear magnetic resonance

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