Abstract
Intrinsic protein fluorescence is due to aromatic amino acids, mainly tryptophan, which can be selectively measured by exciting at 295 nm. Changes in emission spectra of tryptophan are due to the protein conformational transitions, subunit association, ligand binding or denaturation, which affect the local environment surrounding the indole ring. In this study, tryptophan fluorescence was monitored in intact mitochondria at 333 nm following excitation at 295 nm in presence of insecticides using spectrofluorometer. Methyl-parathion, carbofuran, and endosulfan induced Trp fluorescence quenching and release of cytochrome c when incubated with the mitochondria, except fenvalarate. Mechanism of insecticide-induced mitochondrial toxicity for the tested insecticides has been discussed. Reduction in the intensity of tryptophan emission spectra of mitochondrial membrane proteins in presence of an increasing concentration of a ligand can be used to study the interaction of insecticides/drugs with the intact mitochondria. Furthermore, this assay can be readily adapted for studying protein-ligand interactions in intact mitochondria and in other cell organelles extending its implications for pesticide and pharma industry and in drug discovery.
References
May 14, 1975·Journal of the American Chemical Society·D V Bent, E Hayon
Feb 2, 1990·Biochimica Et Biophysica Acta·A J Moreno, V M Madeira
Aug 5, 2000·Environmental Toxicology and Pharmacology·A Wu, Y Liu
Dec 2, 2000·Biochemistry·R LiuF J Sharom
Mar 12, 2003·Toxicology and Applied Pharmacology·Aiguo WuYugu Liu
Jul 2, 2003·Neuroscience·M KitazawaA G Kanthasamy
Apr 10, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M E Chamberlin
Jun 24, 2004·Toxicology·Yoshio NakagawaToshinari Suzuki
Jul 25, 2006·Chemosphere·Antonio J M MorenoVitor M C Madeira
Feb 17, 2007·Journal of Applied Toxicology : JAT·Dan ChenNian Shi
Sep 14, 2007·Neurotoxicology·Pushpinder KaurK D Gill
Mar 15, 2008·Cellular and Molecular Neurobiology·Sukhdev Singh KambojRajat Sandhir
May 27, 2008·Biochimica Et Biophysica Acta·Dirk M Walther, Doron Rapaport
Mar 2, 2010·Biochimica Et Biophysica Acta·Ravindra M AuradeKuruba Sreeramulu
Sep 8, 2010·The Journal of Membrane Biology·Ravindra M AuradeKuruba Sreeramulu
Oct 21, 2010·Chemical Research in Toxicology·M P KashyapA B Pant
May 11, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Muhammad M Hossain, Jason R Richardson
Jan 31, 2012·Journal of Bioenergetics and Biomembranes·S M D AkbarK Sreeramulu
Jan 1, 2012·Archives of Insect Biochemistry and Physiology·Ravindra M AuradeKuruba Sreeramulu
Nov 27, 2014·Cell Biochemistry and Biophysics·Parvez AlamRizwan Hasan Khan
Feb 15, 2015·International Journal of Biological Macromolecules·Basir AhmadRizwan Hasan Khan
Apr 4, 2015·International Journal of Environmental Research and Public Health·Qing LiTomoyuki Kawada
Jun 27, 2015·International Journal of Biological Macromolecules·Sumit Kumar ChaturvediRizwan Hasan Khan
Aug 28, 2015·PloS One·Sumit Kumar ChaturvediRizwan Hasan Khan
Oct 7, 2015·Journal of Biomolecular Structure & Dynamics·Ali S AbdelhameedRizwan Hasan Khan
Dec 1, 2015·International Journal of Biological Macromolecules·Sumit K ChaturvediRizwan H Khan
Citations
Jun 10, 2019·Planta·Yajun LiuTao Xia
Oct 30, 2019·BioFactors·Ali KhattibSoliman Khatib
Apr 10, 2019·Nanoscale·Taegon KimDong June Ahn
Nov 6, 2018·Materials·Faouzi AchouriRaphaël Schneider
Aug 16, 2019·Scientific Reports·Paulina SindrewiczLu-Gang Yu
Jun 5, 2020·Scientific Reports·Narae ShinSeunghun Hong
Jul 13, 2018·Sensors·João Simões, Tao Dong
Aug 31, 2020·Chemistry : a European Journal·Jiang LiuQing Zhu
Aug 21, 2019·Carbohydrate Polymers·Avishek MukherjeeRatan Gachhui
Dec 15, 2020·Antiviral Research·Hanjie Jonathan GanHo Sup Yoon
Nov 10, 2020·International Journal of Biological Macromolecules·Debjani Ghosh, Parnajyoti Karmakar
Jan 21, 2021·Bioorganic & Medicinal Chemistry Letters·Ting ZhuSai-Yang Zhang
Feb 8, 2019·Drug Discovery Today·Sergio C ChaiTaosheng Chen
May 17, 2018·Toxicology Reports·Alexander I TerryGregory K DeKrey
Nov 28, 2020·Pharmacology & Therapeutics·Monimoy BanerjeeBrian S J Blagg
Aug 14, 2021·Bioorganic Chemistry·Ingrid Y Martínez-AldinoJosé Rivera-Chávez
Oct 9, 2018·ACS Chemical Biology·Alescia CullenBrett A Neilan
Oct 26, 2018·Biochemistry·Kanchan AggarwalEmily L Que
Jul 1, 2020·Journal of Natural Products·José Rivera-ChávezPatricia Cano-Sánchez
Feb 18, 2020·Journal of Agricultural and Food Chemistry·Shiqi XuMing Du
Dec 1, 2021·Analytical Chemistry·Chandavalli Ramappa RaghushakerKrishna Kishore Mahato
Jan 7, 2022·ACS Applied Materials & Interfaces·Jin-Young JeongSeunghun Hong