Tryptophan-tryptophan energy migration as a tool to follow apoflavodoxin folding.

Biophysical Journal
Nina V VisserHerbert van Amerongen

Abstract

Submolecular details of Azotobacter vinelandii apoflavodoxin (apoFD) (un)folding are revealed by time-resolved fluorescence anisotropy using wild-type protein and variants lacking one or two of apoFD's three tryptophans. ApoFD equilibrium (un)folding by guanidine hydrochloride follows a three-state model: native <--> unfolded <--> intermediate. In native protein, W128 is a sink for Förster resonance energy transfer (FRET). Consequently, unidirectional FRET with a 50-ps transfer correlation time occurs from W167 to W128. FRET from W74 to W167 is much slower (6.9 ns). In the intermediate, W128 and W167 have native-like geometry because the 50-ps transfer time is observed. However, non-native structure exists between W74 and W167 because instead of 6.9 ns the transfer correlation time is 2.0 ns. In unfolded apoFD this 2.0-ns transfer correlation time is also detected. This decrease in transfer correlation time is a result of W74 and W167 becoming solvent accessible and randomly oriented toward one another. Apparently W74 and W167 are near-natively separated in the folding intermediate and in unfolded apoFD. Both tryptophans may actually be slightly closer in space than in the native state, even though apoFD's radius increases subs...Continue Reading

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Citations

Oct 12, 2012·PloS One·Simon LindhoudCarlo P M van Mierlo
Jul 30, 2009·European Biophysics Journal : EBJ·Bojk A BerghuisHerbert van Amerongen
Jul 21, 2010·Photosynthesis Research·Sashka Boychova KrumovaHerbert van Amerongen
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Jul 12, 2014·Journal of Integrative Neuroscience·Travis J A CraddockJack A Tuszynski
Apr 6, 2017·The FEBS Journal·Joseline A Houwman, Carlo P M van Mierlo
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May 10, 2017·Cellular and Molecular Life Sciences : CMLS·Seyyed Abolghasem GhadamiFabrizio Chiti
Feb 9, 2021·Chembiochem : a European Journal of Chemical Biology·Simone VitielloGlauco Ponterini
Jan 28, 2009·Journal of the American Chemical Society·Sanne M NabuursCarlo P M van Mierlo
May 22, 2009·Journal of the American Chemical Society·Sanne M NabuursCarlo P M van Mierlo

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