Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4

PLoS Neglected Tropical Diseases
Rui ZhangXinping Zhu

Abstract

Trichinellosis is a serious food-borne parasitic zoonosis worldwide. In the effort to develop vaccine against Trichinella infection, we have identified Trichinella spiralis Heat shock protein 70 (Ts-Hsp70) elicits partial protective immunity against T. spiralis infection via activating dendritic cells (DCs) in our previous study. This study aims to investigate whether DCs were activated by Ts-Hsp70 through TLR2 and/or TLR4 pathways. After blocking with anti-TLR2 and TLR4 antibodies, the binding of Ts-Hsp70 to DCs was significantly reduced. The reduced binding effects were also found in TLR2 and TLR4 knockout (TLR2-/- and TLR4-/-) DCs. The expression of TLR2 and TLR4 on DCs was upregulated after treatment with Ts-Hsp70 in vitro. These results suggest that Ts-Hsp70 is able to directly bind to TLR2 and TLR4 on the surface of mouse bone morrow-derived DCs. In addition, the expression of the co-stimulatory molecules (CD80, CD83) on Ts-Hsp70-induced DCs was reduced in TLR2-/- and TLR4-/- mice. More evidence showed that Ts-Hsp70 reduced its activation on TLR2/4 knockout DCs to subsequently activate the naïve T-cells. Furthermore, Ts-Hsp70 elicited protective immunity against T. spiralis infection was reduced in TLR2-/- and TLR4-/- mic...Continue Reading

References

Dec 24, 2002·Current Opinion in Immunology·Shizuo Akira

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Citations

Sep 26, 2020·PLoS Neglected Tropical Diseases·Xuemin JinMingyuan Liu
Jun 27, 2020·Journal of Veterinary Research·Agnieszka Wojtkowiak-GieraEdward Hadaś
Jan 21, 2021·Frontiers in Cellular and Infection Microbiology·Chao YanKui-Yang Zheng
Apr 17, 2019·International Journal of Biological Macromolecules·Mahmoud Abdel-LatifRehab G Khalil

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Methods Mentioned

BETA
gene knockout
Flow Cytometry
ELISA

Software Mentioned

GraphPad Prism

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