TSA restores hair follicle-inductive capacity of skin-derived precursors

Scientific Reports
Ling GuoYaojiong Wu

Abstract

The genesis of the hair follicle relies on signals derived from mesenchymal cells in the dermis during skin morphogenesis and regeneration. Multipotent skin-derived precursors (SKPs), which exhibit long term proliferation potential when being cultured in spheroids, have been shown to induce hair genesis and hair follicle regeneration in mice, implying a therapeutic potential of SKPs in hair follicle regeneration and bioengineering. However, the hair-inductive property of SKPs declines progressively upon ex vivo culture expansion, suggesting that the expressions of the genes responsible for hair induction are epigenetically unstable. In this study, we found that TSA markedly alleviated culture expansion induced SKP senescence, increased the expression and activity of alkaline phosphatase (AP) in the cells and importantly restored the hair inductive capacity of SKPs. TSA increased the acetylation level of histone H3, including the K19/14 sites in the promoter regions of bone morphogenetic proteins (BMPs) genes, which were associated with elevated gene expression and BMP signaling activity, suggesting a potential attribution of BMP pathway in TSA induced recovery of the hair inductive capacity of SKPs.

References

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Citations

Dec 27, 2019·Stem Cells Translational Medicine·Ana Rita Castro, Elsa Logarinho
Sep 3, 2020·Experimental Dermatology·Christos C ZouboulisMarlon R Schneider
Feb 18, 2021·Signal Transduction and Targeted Therapy·Shuaifei JiXiaobing Fu
Apr 22, 2021·The EMBO Journal·Kefei Nina Li, Tudorita Tumbar
Jul 24, 2021·Biochemical and Biophysical Research Communications·Yijie ZhangXiaobing Fu
Dec 24, 2021·Advances in Wound Care·Alvis ChiuFeng Zhao

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Methods Mentioned

BETA
acetylation
PCR
histone acetylation
biopsy
Assay

Software Mentioned

EPSON Scan
Image J

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