TSP-1 Deficiency Alters Ocular Microbiota: Implications for Sjögren's Syndrome Pathogenesis

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
Marielle TerzulliMihaela Gadjeva

Abstract

The potential role of commensals as triggering factors that promote inflammation in dry eye disease has not been explored. The objective of this study was to evaluate whether ocular microbiota changes with the onset of dry eye disease in thrombospondin-1-deficient (TSP-1(-/-)) mice, a strain that develops Sjögren's syndrome-like disease. Conjunctival swabs were collected from TSP-1(-/-) and C57BL/6 mice and analyzed for bacterial presence. Opsonophagocytosis of the bacterial conjunctival isolates derived from the aged TSP-1(-/-) mice by neutrophils derived from either TSP-1(-/-) or C57BL/6 bone marrow was evaluated. The bactericidal activities of TSP-1-derived peptide were examined. We found that in TSP-1(-/-) mice, the conjunctival colonization with Staphylococcus aureus and coagulase negative staphylococci sp (CNS) species was significantly increased with aging and preceded that of the wild-type C57BL/6 control mice. This correlated with increased neutrophil infiltration into the conjunctiva of the TSP-1(-/-) mice, suggesting that TSP-1 plays a significant role in regulating immunity to commensals. Accordingly, the TSP-1(-/-) PMNs opsonophagocytozed the ocular commensals less efficiently than the TSP-1-sufficient neutrophils....Continue Reading

References

Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mihaela GadjevaMichael C Carroll
Oct 29, 2003·The Journal of Biological Chemistry·Rolf WesterbergAnders Jacobsson
Dec 28, 2004·Nature Medicine·Micah T McClainJudith A James
May 30, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Martin MalmstenArtur Schmidtchen
May 18, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Claudia RennemeierBeate E Kehrel
Jul 5, 2007·Clinical Oral Investigations·K C M LeungA S McMillan
Mar 7, 2008·The American Journal of Pathology·Pauline P WardOrla M Conneely
May 6, 2008·Methods in Molecular Biology·Daniel W SiemsenMark T Quinn
Aug 2, 2008·American Journal of Ophthalmology·Yuichi HoriYasuo Tano
Jun 10, 2009·Biochimica Et Biophysica Acta·Adam A StrömstedtMartin Malmsten
Aug 25, 2009·The American Journal of Pathology·Bruce TurpieSharmila Masli
Sep 18, 2010·Investigative Ophthalmology & Visual Science·Julia KlockeMeredith S Gregory
May 17, 2011·Investigative Ophthalmology & Visual Science·Qunfeng DongValery I Shestopalov
May 21, 2013·Cytokine & Growth Factor Reviews·Fabien B VincentFabienne Mackay
Jul 25, 2013·Experimental Eye Research·Alison M McDermott
Nov 13, 2013·Methods in Molecular Biology·Mihaela Gadjeva
Nov 13, 2013·Methods in Molecular Biology·Markryan Dwyer, Mihaela Gadjeva
Mar 19, 2014·Journal of Autoimmunity·Shaye KivityNancy Agmon-Levin
Jun 20, 2014·The Journal of Biological Chemistry·Robert L McKownGordon W Laurie

❮ Previous
Next ❯

Citations

Nov 23, 2018·Experimental Biology and Medicine·Adam D BaimDimitra Skondra
Jul 8, 2016·Nature Reviews. Disease Primers·Pilar Brito-ZerónManuel Ramos-Casals
Jun 27, 2019·Acta Ophthalmologica·Thomas H DohlmanSzilárd Kiss
Dec 4, 2020·International Journal of Molecular Sciences·Sharmila Masli, Darlene A Dartt
Jan 12, 2021·Frontiers in Immunology·Jing Jing LiLai Wei
Apr 6, 2021·Survey of Ophthalmology·Pasquale AragonaMarc Labetoulle

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Scandinavian Journal of Rheumatology. Supplement
F J Holly
Arquivos brasileiros de oftalmologia
Alexandre Mattoso LibórioAna Luísa Höfling de Lima
The British Journal of Ophthalmology
J WilliamsonW C Dick
© 2021 Meta ULC. All rights reserved