tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis.

Genes & Development
Linnan TangPeter N Devreotes

Abstract

In a forward genetic screen for chemotaxis mutants in Dictyostelium discoideum, we identified a loss-of-function mutation, designated tsunami, encoding a homolog of the Fused kinase. Cells lacking tsuA function could not effectively perform chemotaxis and were unable to become polarized or correctly orient pseudopods in chemotactic gradients. While tsuA(-) cells were able to couple receptor occupancy to phosphatidylinositol (3,4,5) trisphosphate (PIP3) production and actin polymerization, the PIP3 response was prolonged and basal F-actin levels were increased. Interestingly, TsuA localizes to the microtubule network and puncta mainly found at the cell periphery. Analysis of the gene uncovered a novel C-terminal domain that we designated the Tsunami Homology (TH) domain. Both the kinase domain and the TH domain are required to rescue the phenotypic defects of tsuA(-) cells. While kinase activity is not required for localization to microtubules, the TH domain is essential. Thus, localization of kinase activity to microtubules is critical for TsuA function. We propose that functions in association with the microtubule network may underlie the divergent roles of Fused kinase proteins in different organisms.

References

Sep 30, 1991·Biochemical and Biophysical Research Communications·L J Wu, P N Devreotes
Jan 1, 1980·Differentiation; Research in Biological Diversity·A Goldbeter, L A Segel
Aug 30, 1983·Biochemical and Biophysical Research Communications·S J McRobbie, P C Newell
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·M UedaU Euteneuer
Jun 14, 2002·Cell·Miho Iijima, Peter Devreotes
Jun 25, 2002·Nature Cell Biology·Orion D WeinerHenry R Bourne
Apr 4, 2003·The Journal of Biological Chemistry·Peter Devreotes, Chris Janetopoulos
Jun 11, 2003·The Journal of Cell Biology·Torsten WittmannClare M Waterman-Storer
Jul 2, 2003·Nature Cell Biology·Olga C RodriguezClare M Waterman-Storer
Sep 4, 2003·Journal of Muscle Research and Cell Motility·David R SollHui Zhang
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Jan 9, 2004·Current Biology : CB·Till BretschneiderGünther Gerisch
Jun 9, 2004·The Journal of Cell Biology·Becket FeierbachFred Chang
Jun 10, 2004·BMC Cell Biology·Andrea Hestermann, Ralph Gräf
Nov 10, 2004·The Journal of Cell Biology·Atsuo T SasakiRichard A Firtel
Apr 29, 2005·Molecular Biology of the Cell·Daniela A BritoMichael P Koonce
Apr 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jingsong XuHenry R Bourne
Oct 20, 2005·Bioinformatics·Ezgi O BoothGad Shaulsky
Feb 7, 2006·Developmental Cell·Markku VarjosaloJussi Taipale
Mar 18, 2006·Thrombosis and Haemostasis·Anna BagordaCarole A Parent
Jun 20, 2006·Current Opinion in Genetics & Development·Jonathan Franca-KohPeter Devreotes
Sep 8, 2006·Methods in Molecular Biology·Adam Kuspa
Sep 12, 2006·European Journal of Cell Biology·Stacey S Willard, Peter N Devreotes
Dec 22, 2006·Journal of Cell Science·Markku Varjosalo, Jussi Taipale
Dec 30, 2006·Current Opinion in Cell Biology·Roshni Basu, Fred Chang
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jingsong XuHenry R Bourne
Nov 28, 2007·BMC Biology·Cathryn KabacoffDouglas N Robinson
Jan 18, 2008·Biophysical Journal·Javier SatulovskyYu-li Wang
Jan 22, 2008·Current Opinion in Cell Biology·Pablo A Iglesias, Peter N Devreotes

❮ Previous
Next ❯

Citations

May 19, 2011·Development, Growth & Differentiation·Yu WangMiho Iijima
Mar 3, 2010·Annual Review of Biophysics·Kristen F SwaneyPeter N Devreotes
Jun 10, 2010·Development·Christopher W Wilson, Pao-Tien Chuang
Apr 23, 2009·PloS One·Leonard Bosgraaf, Peter J M Van Haastert
Oct 23, 2013·Plant Reproduction·Sung Aeong OhSoon Ki Park
May 23, 2014·Cellular and Molecular Life Sciences : CMLS·Yulia ArtemenkoPeter N Devreotes
Aug 7, 2010·Trends in Cell Biology·Rita TewariJuliet C Coates
Jun 20, 2012·The Plant Journal : for Cell and Molecular Biology·Sung Aeong OhDavid Twell
Aug 3, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yoko Inès NozawaPao-Tien Chuang
May 20, 2009·Biophysical Journal·Daniel IrimiaMehmet Toner
Apr 9, 2010·Molecular Biology of the Cell·Susan LeeRichard A Firtel
Feb 22, 2011·Biochimica Et Biophysica Acta·S de KeijzerP A Iglesias
Apr 20, 2011·Nature Reviews. Genetics·Philip W InghamClaudia Seger
Oct 23, 2021·Frontiers in Cell and Developmental Biology·Jonathan KuhnPeter N Devreotes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.