TTF-2 stimulates expression of 17 genes, including one novel thyroid-specific gene which might be involved in thyroid development

Molecular and Cellular Endocrinology
Akira HishinumaTamio Ieiri

Abstract

Thyroid dysgenesis is the most frequent cause of congenital hypothyroidism, but its molecular pathophysiology is largely unknown. Our hypothesis that some genes downstream to thyroid transcription factor-2 (TTF-2) might be responsible for development of the thyroid prompted us to identify genes whose expression is stimulated by TTF-2. PCR products of cDNA clones obtained by a subtraction PCR method in TTF-2 expressing cell lines were screened with labeled cDNA by microarray analysis. We isolated 17 genes up-regulated by TTF-2, which were subsequently confirmed by quantitative reverse transcription-polymerase chain reaction (RT-PCR). One of them is a novel gene designated T1560 that showed a highly thyroid-specific expression pattern. Luciferase reporter assays showed that expression of all of the 14 genes tested was stimulated by both TTF-2 and TTF-1, another thyroid-specific transcription factor. Our results have important implications for understanding normal thyroid development as well as the molecular defects underlying thyroid dysgenesis.

References

Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Kozak
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 19, 1995·The New England Journal of Medicine·T SunthornthepvarakulS Refetoff
Apr 20, 1995·The New England Journal of Medicine·H WatkinsJ G Seidman
Aug 11, 1998·Nature Genetics·M De FeliceR Di Lauro
Oct 30, 1998·American Journal of Human Genetics·A ShanskeR W Marion
Aug 14, 1999·Thyroid : Official Journal of the American Thyroid Association·S LaFranchi
Aug 15, 2002·The Journal of Biological Chemistry·Claudia FolliRodolfo Berni

❮ Previous
Next ❯

Citations

Dec 24, 2010·Human Molecular Genetics·Isabella VenzaMario Venza
Oct 18, 2011·BMC Evolutionary Biology·Sergey Yu YaklichkinSridhar Hannenhalli
Dec 31, 2014·Archives of Pathology & Laboratory Medicine·Haiyan Liu, Fan Lin
Oct 29, 2014·Nature Reviews. Endocrinology·Lara P FernándezPilar Santisteban
Mar 1, 2006·Expert Review of Endocrinology & Metabolism·John S Fuqua
Nov 10, 2020·Frontiers in Immunology·Chanan MeydanHermona Soreq

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alpha-Synuclein Aggregation (MDS)

Alpha-synucleins are small proteins that are believed to restrict the mobility of synpatic vesicles and inhibit neurotransmitter release. Aggregation of these proteins have been linked to several types of neurodegenerative diseases including dementia with Lewy bodies and Parkinson's disease. Here is the latest research on α-synuclein aggregation.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.