Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish.

Molecular and Cellular Neurosciences
Matthew B VeldmanD Goldman

Abstract

We report that knockdown of the alpha1 tubulin isoform Tuba1a, but not the highly related Tuba1b, dramatically impedes nervous system formation during development and RGC axon regeneration following optic nerve injury in adults. Within the tuba1a promoter, a G/C-rich element was identified that is necessary for tuba1a induction during RGC differentiation and optic axon regeneration. KLF6a and 7a, which we previously reported are essential for optic axon regeneration (Veldman et al., 2007), bind this G/C-rich element and transactivate the tuba1a promoter. In vivo knockdown of KLF6a and 7a attenuate regeneration-dependent activation of the endogenous tuba1a and p27 genes. These results suggest tuba1a expression is necessary for CNS development and regeneration and that KLF6a and 7a mediate their effects, at least in part, via transcriptional control of tuba1a promoter activity.

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Citations

Jul 13, 2013·Neuroscience Bulletin· SaijilafuFeng-Quan Zhou
Jan 21, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Curtis PowellDaniel Goldman
Oct 18, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Lisbeth CubillánLucimey Lima
Sep 14, 2010·Current Opinion in Neurobiology·Zhiping Wang, Yishi Jin
Apr 27, 2010·Current Opinion in Neurobiology·Fang Sun, Zhigang He
Mar 5, 2016·Experimental Neurology·Jeffrey P Rasmussen, Alvaro Sagasti
May 20, 2011·Developmental Neurobiology·Gaia GestriStephan C F Neuhauss
Apr 29, 2014·Current Opinion in Neurobiology·Thomas Becker, Catherina G Becker
Jul 2, 2016·Gene Expression Patterns : GEP·Sunil BhattaraiQin Liu
Oct 24, 2017·Journal of Developmental Biology·Jayne AikenJeffrey K Moore
Jul 22, 2018·Developmental Neurobiology·Sahil H Shah, Jeffrey L Goldberg
Feb 1, 2012·Journal of Tissue Engineering·Toby A Ferguson, Young-Jin Son
May 4, 2018·Regeneration·Sukla Ghosh, Subhra Prakash Hui
May 23, 2018·Scientific Reports·Melissa FariaDemetrio Raldúa

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