TUBB4B Downregulation Is Critical for Increasing Migration of Metastatic Colon Cancer Cells

Cells
Katarzyna SobierajskaM Anna Kowalska

Abstract

Tumor metastasis, the major problem for clinical oncology in colon cancer treatment, is linked with an epithelial-mesenchymal transition (EMT). The observed cellular transformation in this process is manifested by cell elongation, enhanced cell migration and invasion ability, coordinated by cytoskeleton reorganization. In the present study, we examined the role of tubulin-β4 (TUBB4B) downregulation that occurs during EMT in colon cancer cells, in the modulation of the function of microtubules. Based on biochemical and behavioral analysis (transmigration) we posit that the decrease of the TUBB4B level is critical for microtubule-vimentin interaction and contributes to the maintenance of polarity in migrating cells. The microscopic studies revealed that TUBB4B decrease is accompanied by cell elongation and increased number of matured focal adhesion sites, which is a characteristic of the cell metastatic stage. We also demonstrated faster polymerization of microtubules in cells with a lower level of TUBB4B. Simultaneous TUBB3 upregulation, reported during EMT, acts additively in this process. Our studies suggest that the protein level of TUBB4B could be used as a marker for detection of the preinvasive stages of the colon cancer c...Continue Reading

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Citations

Apr 8, 2020·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Chie FurihataTakayoshi Suzuki
Apr 3, 2020·International Journal of Molecular Medicine·Chunlei YuanSijia Duan
Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Filip BorysHanna Fabczak
Jan 29, 2021·Journal of Cellular Physiology·Arghavan ZebardastFarzin Sadeghi
Feb 3, 2021·Photodiagnosis and Photodynamic Therapy·Haixiu MaLing Kang
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Laura Maeso-AlonsoMaria C Marin

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Methods Mentioned

BETA
PCR
Protein Assay
immunoprecipitation
Assay
Glycosylation
immunoprecipitation assay
transfection
confocal microscopy
co-immunoprecipitation

Software Mentioned

GraphPad Prism
ImageJ
LAS AF

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