"Tuberculosis in advanced HIV infection is associated with increased expression of IFNγ and its downstream targets"

BMC Infectious Diseases
Sheetal VermaPadmini Salgame

Abstract

Tuberculosis (TB) is the major cause of death in Human Immunodeficiency Virus (HIV)-infected individuals. However, diagnosis of TB in HIV remains challenging particularly when HIV infection is advanced. Several gene signatures and serum protein biomarkers have been identified that distinguish active TB from latent infection. Our study was designed to assess if gene expression signatures and cytokine levels would distinguish active TB in advanced HIV. We conducted a case-control study of whole blood RNA-Seq and plasma cytokine/chemokine analysis in HIV-infected with CD4+ T cell count of ≤ 100 cells/μl, with and without active TB. Next, the overlap of the differentially expressed genes (DEG) with the published signatures was performed and then receiver operator characteristic (ROC) analysis was done on small gene discriminators to determine their performance in distinguishing TB in advanced HIV. ELISA was performed on plasma to evaluate cytokine and chemokine levels. Hierarchical clustering of the transcriptional profiles showed that, in general, HIV-infected individuals with TB (TB-HIV) clustered separately from those without TB. IPA indicated that the TB-HIV signature was characterized by an increase in inflammatory signaling p...Continue Reading

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Citations

Sep 27, 2018·The Journal of Gene Medicine·Guoxia RenMingwei Chen
Nov 26, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Yukari C ManabeAmita Gupta
Oct 5, 2018·Current Opinion in HIV and AIDS·Elsa du BruynRobert J Wilkinson
Jan 15, 2021·Genome Medicine·Anna C AschenbrennerUNKNOWN German COVID-19 Omics Initiative (DeCOI)
Jan 24, 2021·BMC Infectious Diseases·W Evan JohnsonNatasha S Hochberg
Oct 29, 2020·Scientific Reports·Marta TagliabueMohssen Ansarin
Mar 12, 2021·Frontiers in Immunology·Vandana KulkarniVidya Mave

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Methods Mentioned

BETA
metabolomic profiling
PCR
ELISA
RNA-Seq
PCA

Software Mentioned

Ensembl
FastQC
R
STAR
pROC
gplots
Bioconductor DESeq
MSD DISCOVERY WORKBENCH®
Cutadapt
TopHat

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