PMID: 9548366Apr 21, 1998Paper

Tubular peroxisomes in HepG2 cells: selective induction by growth factors and arachidonic acid

European Journal of Cell Biology
Michael SchraderH Dariush Fahimi

Abstract

We showed recently the plasticity of the peroxisomal compartment in the human hepatoblastoma cell line HepG2 as evidenced by the presence of elongated tubular peroxisomes measuring up to 5 microm next to much smaller spherical or rod-shaped ones (0.1-0.3 microm). Since the occurrence of tubular peroxisomes in a given cell in culture is synchronized, with neighboring cells containing either small spherical or elongated tubular peroxisomes, cell counting of immunofluorescence preparations stained for catalase was used for the quantitative assessment of the dynamics of the peroxisomal compartment and the factors regulating this process. Initial studies revealed that the formation of tubular peroxisomes is primarily influenced by the cell density as well as by lipid- and protein-factors in fetal calf serum, being independent of an intact microtubular network. Biochemical studies showed that the occurrence of tubular peroxisomes correlated with the expression of the mRNA for 70 kDa peroxisomal membrane protein (PMP70), but not with that of matrix proteins. By cultivation of cells in serum- and protein-free media specific factors were identified which influenced the formation of tubular peroxisomes. Among several growth factors teste...Continue Reading

References

May 1, 1975·Journal of Ultrastructure Research·F P Wedel, E R Berger
Jun 1, 1979·Scientific American·R Levi-Montalcini, P Calissano
Mar 6, 1992·Cell·I Mellman, K Simons
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Feb 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J H Kelly, G J Darlington
Oct 1, 1986·The Journal of Cell Biology·M TerasakiK Fujiwara
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SwansonS C Silverstein
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L V JohnsonL B Chen
Jan 1, 1982·Annals of the New York Academy of Sciences·A K Hajra, J E Bishop
Jul 1, 1995·Molecular Biology of the Cell·P de Figueiredo, W J Brown
Apr 1, 1995·The Journal of Cell Biology·P A MarshallJ M Goodman
Apr 1, 1995·Journal of Neurochemistry·C Lomen-Hoerth, E M Shooter
Aug 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M LindauerH D Fahimi
Jan 1, 1994·Annual Review of Nutrition·J K Reddy, G P Mannaerts
Aug 1, 1994·Biology of Reproduction·D DjakiewM Onoda
Jun 1, 1994·Journal of Neurochemistry·D SampathJ R Perez-Polo
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H KellerW Wahli
Apr 1, 1996·The Journal of Nutrition·S D Clarke, D B Jump
Mar 1, 1996·Cellular Signalling·C J Masters
Aug 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A SchadE Baumgart
Oct 1, 1996·The Journal of Cell Biology·B DistelM Veenhuis
Oct 1, 1996·The Journal of Cell Biology·P A MarshallJ M Goodman
Jun 1, 1993·Current Biology : CB·A W Shyjan, R A Buttow

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Citations

Jul 9, 2004·Histochemistry and Cell Biology·Michael Schrader, H Dariush Fahimi
Mar 15, 2012·Histochemistry and Cell Biology·Markus IslingerMichael Schrader
Apr 26, 2000·Biochimica Et Biophysica Acta·A CiminiM P Cerù
Jun 28, 2005·Current Opinion in Cell Biology·Mingda YanSuresh Subramani
Jan 14, 2012·Biochimica Et Biophysica Acta·M SchraderM Islinger
Nov 28, 2000·Annual Review of Genetics·K A Sacksteder, S J Gould
Feb 21, 2006·Biochimica Et Biophysica Acta·Michael Schrader
Dec 2, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Helena OliveiraConceição Santos
Oct 13, 2006·Biochimica Et Biophysica Acta·Michael Schrader, H Dariush Fahimi
Jan 12, 2013·PloS One·Nina A BonekampMichael Schrader
May 20, 2017·Histochemistry and Cell Biology·Josiah B PassmoreMichael Schrader
Oct 29, 1998·The Journal of Biological Chemistry·M SchraderS J Gould
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M SchraderH D Fahimi
Oct 23, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Schrader
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H D Fahimi, E Baumgart
Aug 31, 2007·The Journal of Comparative Neurology·Barbara AhlemeyerEveline Baumgart-Vogt
May 25, 2007·The Journal of Biological Chemistry·Markus IslingerAlfred Völkl
Dec 25, 2002·The Journal of Biological Chemistry·Annett KochMichael Schrader
May 13, 2003·Microscopy Research and Technique·Michael SchraderH Dariush Fahimi
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Afsoon S AzadiMichael Schrader

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