Tubulation repair mitigates misdirection of regenerating motor axons across a sciatic nerve gap in rats

Scientific Reports
Dan LiuWen Hu

Abstract

The repair of peripheral nerve laceration injury to obtain optimal function recovery remains a big challenge in the clinic. Misdirection of regenerating axons to inappropriate target, as a result of forced mismatch of endoneurial sheaths in the case of end-to-end nerve anastomosis or nerve autografting, represents one major drawback that limits nerve function recovery. Here we tested whether tubulation repair of a nerve defect could be beneficial in terms of nerve regeneration accuracy and nerve function. We employed sequential retrograde neuronal tracing to assess the accuracy of motor axon regeneration into the tibial nerve after sciatic nerve laceration and entubulation in adult Sprague-Dawley rats. In a separate cohort of rats with the same sciatic nerve injury/repair protocols, we evaluated nerve function recovery behaviorally and electrophysiologically. The results showed that tubulation repair of the lacerated sciatic nerve using a 3-6-mm-long bioabsorbable guidance conduit significantly reduced the misdirection of motor axons into the tibial nerve as compared to nerve autografting. In addition, tubulation repair ameliorated chronic flexion contracture. This study suggests that tubulation repair of a nerve laceration inj...Continue Reading

References

Jan 1, 1989·Progress in Neurobiology·R D FieldsM H Ellisman
Jul 27, 2002·Annals of Plastic Surgery·Romed MeirerMaria Siemionow
Mar 11, 2004·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·G LundborgI Rosén
Jul 8, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lee J MartinZhiping Liu
Sep 22, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·A HökeT M Brushart
Jan 26, 2007·Acta Physiologica·R D MadisonS R Chadaram
Jan 26, 2007·Acta Physiologica·G Lundborg, B Rosén
Feb 21, 2008·Microsurgery·Przemyslaw LubiatowskiMaria Siemionow
Jul 10, 2008·Biotechnology Letters·Wen HuXiaosong Gu
Nov 15, 2008·Experimental Neurology·Grant A Robinson, Roger D Madison
Jan 13, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Baoguo JiangBailin Jiang
Jan 19, 2010·Experimental Neurology·John W GriffinMohamed H Farah
Dec 2, 2010·The Journal of Comparative Neurology·Shirley K HamiltonArthur W English
Mar 4, 2011·Journal of Tissue Engineering and Regenerative Medicine·Jianhui GuXiaosong Gu
Jan 21, 2014·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·S KambizE T Walbeehm
Nov 15, 2014·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Yaxian WangWen Hu
Jun 21, 2015·Progress in Neurobiology·Valeria Chiono, Chiara Tonda-Turo

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Citations

Nov 13, 2020·Frontiers in Bioengineering and Biotechnology·Benedetta E FornasariStefania Raimondo
Feb 13, 2021·International Journal of Molecular Sciences·Rui D AlvitesAna C Maurício
Jul 6, 2019·ACS Chemical Neuroscience·Shadi HoushyarRobert Shanks

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BETA
confocal microscopy

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