PMID: 7026576Sep 1, 1981Paper

Tubulin assembly sites and the organization of cytoplasmic microtubules in cultured mammalian cells

The Journal of Cell Biology
B R BrinkleyR L Pardue

Abstract

The number, distribution, and nucleating capacity of microtubule-organizing centers (MTOCs) has been investigated in a variety of cultured mammalian cells. Most interphase cells contain a single MTOC that is localized at the centrosome region and corresponds to the centriole and pericentriolar material. MTOCs, like centrioles, become duplicated during the S phase of the cell cycle and are equationally distributed to daughter cells in mitosis. Multiple MTOCs were rarely observed in cultured cells except in one cell line (neuroblastoma), which also displayed an equally large number of centrioles in the cytoplasm. The kinetics of microtubule assembly and the tubulin nucleating capacity of MTOCs was assayed by incubating tubulin-depleted, permeabilized 3T3 and simian virus 40-transformed 3T3 cells with phosphocellulose-purified 65 brain tubulin and microtubule assembly buffer. Initiation and assembly of 65 tubulin occurred in association with the cells' endogenous MTOCs, and the length, number, and distribution of microtubules generated about the organizing centers were regulated and cell specific. Our results are consistent with the notion that the specification of microtubule length, number, and spatial arrangement resides largel...Continue Reading

References

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Citations

Dec 19, 1998·Cell Motility and the Cytoskeleton·B R Brinkley, T M Goepfert
Jul 13, 2000·Cell Motility and the Cytoskeleton·E Frixione
Dec 18, 2001·Cell Motility and the Cytoskeleton·B A DanowskiP Wadsworth
Nov 9, 2004·Cell Motility and the Cytoskeleton·Elisabeth BugnardEvelyn Ralston
Jan 22, 2009·Cancer Metastasis Reviews·Susana A GodinhoDavid Pellman
Jan 1, 1985·The International Journal of Biochemistry·J E Hesketh
Mar 16, 1984·Journal of Immunological Methods·P Kurki, I Virtanen
Jan 1, 1984·Pharmacology & Therapeutics·J J Manfredi, S B Horwitz
Jan 1, 1983·Experimental Gerontology·M RaesJ Remacle
Nov 5, 2002·Nature Reviews. Cancer·Erich A Nigg
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Caplow, R Reid
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G G Gundersen, J C Bulinski
Jan 1, 1985·The Journal of Cell Biology·R B Nicklas, G W Gordon
Apr 1, 1985·The Journal of Cell Biology·L EvansM Kirschner
Jun 1, 1995·The Journal of Cell Biology·D Zhang, R B Nicklas
Sep 1, 1981·The Journal of Cell Biology·F Solomon
Sep 1, 1982·The Journal of Cell Biology·D RingM Kirschner
Jun 1, 1983·The Journal of Cell Biology·W J Deery, B R Brinkley
Jul 1, 1984·The Journal of Cell Biology·J B OlmstedH D Lyon
Jul 1, 1984·The Journal of Cell Biology·J B Tucker
Dec 1, 1984·The Journal of Cell Biology·W M SaxtonJ R McIntosh
Feb 1, 1983·British Journal of Haematology·J M Radley, C J Haller
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S A Godinho, D Pellman
Dec 9, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Maddalena Nano, Renata Basto
May 26, 2009·Toxicon : Official Journal of the International Society on Toxinology·Dániel BeyerCsaba Máthé

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