PMID: 7541636Jun 1, 1995Paper

Tubulin dynamics in neuronal axons of living zebrafish embryos

Neuron
S TakedaN Hirokawa

Abstract

The mechanism of cytoskeletal protein transport, especially the question of what kind of form the cytoskeletal proteins assume during transport in neurons in situ, has been an important, as yet unsettled issue. To clear up this matter, we adopted the embryonic zebrafish as a living animal model and applied the fluorescence recovery after photobleaching (FRAP) method. The zebrafish embryo is appropriate for this kind of study because of its transparency during the early developmental stage, allowing the observation of neurons that incorporate the microinjected fluorescent tubulin directly under fluorescence microscopy. FRAP revealed no movement of the bleached zone proximodistally, where fluorescence recovered gradually (recovery half-time, 44.2 +/- 11.2 min; n = 36), suggesting that the polymers are stationary but dynamic and that the true moving form could be small oligomers or heterodimers.

References

Feb 1, 1992·Current Opinion in Cell Biology·R A Nixon
Jan 1, 1992·Cell Motility and the Cytoskeleton·R A Nixon, T B Shea
Dec 15, 1990·The Journal of Comparative Neurology·R R BernhardtJ Y Kuwada
Oct 1, 1991·The Journal of Cell Biology·S S ReinschM Kirschner
Oct 1, 1991·The Journal of Cell Biology·E M Tanaka, M W Kirschner
Jul 1, 1990·The Journal of Cell Biology·S S LimG G Borisy
Dec 15, 1990·The Journal of Comparative Neurology·J Y KuwadaN Nguyen
Jan 1, 1986·Journal of Cell Science. Supplement·R J Lasek
Sep 1, 1988·The Journal of Cell Biology·G P VigersJ R McIntosh
Aug 1, 1980·The Journal of Cell Biology·M M Black, R J Lasek
Oct 1, 1980·Physiological Reviews·B Grafstein, D S Forman
Oct 1, 1994·The Journal of Cell Biology·S TakedaN Hirokawa
Oct 1, 1993·Current Opinion in Neurobiology·N Hirokawa
Apr 1, 1993·The Journal of Cell Biology·S OkabeN Hirokawa
Apr 1, 1992·Current Biology : CB·N Hirokawa, S Okabe

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Citations

Apr 1, 1996·Molecular Neurobiology·P W Baas, W Yu
May 2, 2006·Journal of Computational Neuroscience·Bruce P GrahamDouglas R Mclean
Aug 22, 2007·Trends in Cell Biology·N HirokawaS Takeda
Aug 22, 2007·Trends in Cell Biology·P W Baas, A Brown
Apr 1, 1996·The Journal of Cell Biology·W YuP W Baas
Jun 1, 1996·The Journal of Cell Biology·K E Miller, H C Joshi
Jun 29, 2004·Cell Structure and Function·C BoscD Job
Oct 6, 1999·The Journal of Cell Biology·H L ZhangG J Bassell
Aug 8, 2009·Molecular and Cellular Neurosciences·Fabienne E Poulain, André Sobel
Jul 13, 2000·The Journal of Cell Biology·S AckerleyC C Miller
Feb 11, 1999·The Journal of Cell Biology·T J Koehnle, A Brown
Aug 26, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kazuhide Asakawa, Koichi Kawakami
Mar 20, 2001·Nature Reviews. Molecular Cell Biology·A Brown
Mar 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G J BassellR H Singer
Apr 5, 2021·Neuroscience Letters·Shrobona GuhaPeter W Baas
Mar 4, 1998·Current Opinion in Cell Biology·H C Joshi
Sep 13, 2002·Current Biology : CB·Lei Wang, Anthony Brown

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