Tubulin polyglycylation in Platyhelminthes: diversity among stable microtubule networks and very late occurrence during spermiogenesis

Cell Motility and the Cytoskeleton
C IominiJ L Justine

Abstract

The distribution of glycylated tubulin has been analyzed in different populations of stable microtubules in a digenean flatworm, Echinostoma caproni (Platyhelminthes). Two cellular types, spermatozoa and ciliated excretory cells, have been analyzed by means of immunofluorescence, immunogold, and immunoblotting techniques using two monoclonal antibodies (mAbs), AXO 49, and TAP 952, specifically directed against differently glycylated isoforms of tubulin. The presence of glycylated tubulin in the two cell types was shown. However, the differential reactivities of TAP 952 and AXO 49 mAbs with the two axoneme types suggest a difference in their glycylation level. In addition, within a single cell, the spermatozoon, cortical microtubules underlying the flagellar membrane, and axonemal microtubules were shown to comprise different tubulin isoforms, the latter ones only being labelled with one of the antiglycylated tubulin mAbs, TAP 952. Similarly, the antiacetylated (6-11B-1) and polyglutamylated (GT335) tubulin mAbs decorated the two types of axonemal microtubules, but not the cortical ones. From these data, a subcellular sorting of posttranslationally modified tubulin isoforms within spermatozoa, on the one hand, and a cellular sor...Continue Reading

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Citations

Mar 17, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Behrokh Mohajer-MaghariImogen R Coe
Jun 29, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Malcolm K JonesDonald P McManus
May 18, 2004·Cell Motility and the Cytoskeleton·Caterina MencarelliRomano Dallai
Jul 1, 2005·Cell Motility and the Cytoskeleton·Caterina MencarelliRomano Dallai
Oct 3, 2003·Biochimica Et Biophysica Acta·Francesca SimoncelliRita Pascolini

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