Tudor-SN Interacts with Piwi Antagonistically in Regulating Spermatogenesis but Synergistically in Silencing Transposons in Drosophila

PLoS Genetics
Hsueh-Yen KuHaifan Lin

Abstract

Piwi proteins associate with piRNAs and functions in epigenetic programming, post-transcriptional regulation, transposon silencing, and germline development. However, it is not known whether the diverse functions of these proteins are molecularly separable. Here we report that Piwi interacts with Tudor-SN (Tudor staphylococcal nuclease, TSN) antagonistically in regulating spermatogenesis but synergistically in silencing transposons. However, it is not required for piRNA biogenesis. TSN is known to participate in diverse molecular functions such as RNAi, degradation of hyper-edited miRNAs, and spliceosome assembly. We show that TSN colocalizes with Piwi in primordial germ cells (PGCs) and embryonic somatic cells. In adult ovaries and testes, TSN is ubiquitously expressed and enriched in the cytoplasm of both germline and somatic cells. The tsn mutants display a higher mitotic index of spermatogonia, accumulation of spermatocytes, defects in meiotic cytokinesis, a decreased number of spermatids, and eventually reduced male fertility. Germline-specific TSN-expression analysis demonstrates that this function is germline-dependent. Different from other known Piwi interters, TSN represses Piwi expression at both protein and mRNA leve...Continue Reading

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Citations

Oct 19, 2016·Cell Death and Differentiation·Emilio Gutierrez-BeltranPeter V Bozhkov
May 8, 2018·The Journal of Biological Chemistry·Rasesh Y ParikhVamsi K Gangaraju
May 14, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Michael K SkinnerJohn R McCarrey
Nov 16, 2019·Aging·Ye ChengWeihong Shi
May 31, 2020·Fish Physiology and Biochemistry·Weidong DingXuwen Bing
Apr 23, 2021·Interdisciplinary Sciences, Computational Life Sciences·Yajun LiuXinhong Hei
Feb 15, 2020·IScience·Sarah Hélène MerklingLouis Lambrechts
Oct 11, 2020·Mechanisms of Development·Antonio L Rockwell, Cintia F Hongay

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Methods Mentioned

BETA
co-immunoprecipitation
co-immunoprecipitations
phase
immuneprecipitation
transgenic
Immunoprecipitation

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