TULA proteins regulate activity of the protein tyrosine kinase Syk.

Journal of Cellular Biochemistry
Rachana AgrawalAlexander Y Tsygankov

Abstract

TULA belongs to a two-member family: TULA (STS-2) is a lymphoid protein, whereas STS-1/TULA-2 is expressed ubiquitously. TULA proteins were implicated in the regulation of signaling mediated by protein tyrosine kinases (PTKs). The initial experiments did not fully reveal the molecular mechanism of these effects, but suggested that both TULA proteins act in a similar fashion. It was shown recently that STS-1/TULA-2 dephosphorylates PTKs. In this study, we analyzed the effects of TULA proteins on Syk, a PTK playing an important role in lymphoid signaling. First, we have shown that TULA-2 decreases tyrosine phosphorylation of Syk in vivo and in vitro and that the intact phosphatase domain of TULA-2 is essential for this effect. We have also shown that TULA-2 exhibits a certain degree of substrate specificity. Our results also indicate that inactivated TULA-2 increases tyrosine phosphorylation of Syk in cells co-transfected to overexpress these proteins, thus acting as a dominant-negative form that suppresses dephosphorylation of Syk caused by endogenous TULA-2. Furthermore, we have demonstrated that phosphatase activity of TULA is negligible as compared to that of TULA-2 and that this finding correlates with an increase in Syk tyr...Continue Reading

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Citations

Jul 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kohsuke TakedaHidenori Ichijo
Jan 10, 2009·Annual Review of Immunology·Jennifer E Smith-GarvinMartha S Jordan
Jul 22, 2011·Journal of Signal Transduction·Rodrigo Orlandini de Castro
May 16, 2012·The Journal of Clinical Investigation·Jian HongTiebang Kang
Jul 9, 2009·Cellular and Molecular Life Sciences : CMLS·Alexander Y Tsygankov
Nov 15, 2012·Cellular and Molecular Life Sciences : CMLS·Steven H BackArchana Sanjay
Nov 18, 2015·The FEBS Journal·Andrés Alonso, Rafael Pulido
May 12, 2015·European Journal of Immunology·Guanjun DongYayi Hou
Jun 13, 2012·Journal of Cellular Physiology·Alexander Y Tsygankov
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Mar 25, 2009·Biochimica Et Biophysica Acta·Robert L Geahlen
Feb 28, 2013·International Journal of Molecular Sciences·Daichi SadatomiKohsuke Takeda
Oct 4, 2017·Modern Rheumatology·Namiko OkabeTsuneyo Mimori
Aug 5, 2018·Journal of Cellular Physiology·Alexander Y Tsygankov
Oct 30, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yan GePatrick Concannon
Aug 26, 2020·Immunity, Inflammation and Disease·Kaustubh Parashar, Nicholas Carpino
Mar 5, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Edwina Naik, Vishva M Dixit

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